Tested Applications
| Positive WB detected in | HEK-293 cells, A549 cells, HepG2 cells, HeLa cells, Raji cells, MCF-7 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells, MCF-7 cells |
| Positive FC (Intra) detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83608-5-RR targets ATM in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag25920 Product name: Recombinant human ATM protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 2407-2709 aa of NM_000051 Sequence: SSEFENKQALLKRAKEEVGLLREHKIQTNRYTVKVQRELELDELALRALKEDRKRFLCKAVENYINCLLSGEEHDMWVFRLCSLWLENSGVSEVNGMMKRDGMKIPTYKFLPLMYQLAARMGTKMMGGLGFHEVLNNLISRISMDHPHHTLFIILALANANRDEFLTKPEVARRSRITKNVPKQSSQLDEDRTEAANRIICTIRSRRPQMVRSVEALCDAYIILANLDATQWKTQRKGINIPADQPITKLKNLEDVVVPTMEIKVDHTGEYGNLVTIQSFKAEFRLAGGVNLPKIIDCVGSDG Predict reactive species |
| Full Name | ataxia telangiectasia mutated |
| Observed Molecular Weight | 310-350 kDa |
| GenBank Accession Number | NM_000051 |
| Gene Symbol | ATM |
| Gene ID (NCBI) | 472 |
| RRID | AB_3671222 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13315 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The ATM protein is a member of the phosphatidylinositol 3-kinase family of proteins that respond to DNA damage by phosphorylating key substrates involved in DNA repair and/or cell cycle control. It is involved in mitogenic signal transduction, meiotic recombination, detection of DNA damage, and cell cycle control.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for ATM antibody 83608-5-RR | Download protocol |
| IF protocol for ATM antibody 83608-5-RR | Download protocol |
| IHC protocol for ATM antibody 83608-5-RR | Download protocol |
| WB protocol for ATM antibody 83608-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ATM Recombinant monoclonal antibody (240560F5), catalog number 83608-5-RR, has 3 total citations. These appear in Drug Resistance Updates, Biochemical Pharmacology, and Inflammation Research. The associated research fields span pancreatic ductal adenocarcinoma (alternative splicing and chemo/immunotherapy sensitization), renal fibrosis (SGLT2 inhibitor–induced senescence and EMT), and osteoarthritis (IL-4/STAT6-regulated macrophage efferocytosis).
| Species | Application | Title |
|---|---|---|
Drug Resist Updat Targeting CLK1/SRSF7 axis-dependent alternative splicing sensitizes pancreatic ductal adenocarcinoma to chemotherapy and immunotherapy | ||
Biochem Pharmacol SGLT2i-Triggered hyperglycosuria Unveils CDKN2A-Driven distal tubular senescence and EMT, Fueling renal fibrosis.
| ||
Inflamm Res The IL-4/IL-4R/STAT6 signaling axis regulates macrophage efferocytosis in osteoarthritis. |
