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ATN1 Polyclonal antibody

ATN1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19243-1-AP

Synonyms

ATN1, atrophin 1, B37, D12S755E, DRPLA, HRS, NOD

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using ATN1 Polyclonal antibody (19243-1-AP)

    WB analysis of mouse testis using 19243-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 19243-1-AP (ATN1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tissue using ATN1 Polyclonal antibody (19243-1-AP)

    IHC staining of human ovary using 19243-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 19243-1-AP (ATN1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using ATN1 Polyclonal antibody (19243-1-AP)

    IHC staining of human ovary using 19243-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 19243-1-AP (ATN1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ATN1 Polyclonal antibody (19243-1-AP)

    IHC staining of human testis using 19243-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19243-1-AP (ATN1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ATN1 Polyclonal antibody (19243-1-AP)

    IHC staining of human testis using 19243-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19243-1-AP (ATN1 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman ovary tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 19243-1-AP is ATN1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATN1 fusion protein Ag5835
Full Name atrophin 1
Calculated Molecular Weight 125 kDa
Observed Molecular Weight 105-110 kDa
GenBank Accession NumberBC051795
Gene Symbol ATN1
Gene ID (NCBI) 1822
RRIDAB_2878575
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATN1 also known as Atrophin-1 is a protein that in humans is encoded by the ATN1 gene. Dentatorubral pallidoluysian atrophy is a rare neurodegenerative disorder characterized by cerebellar ataxia, myoclonic epilepsy, choreoathetosis, and dementia. The disorder is related to the expansion of a trinucleotide repeat within this gene. The encoded protein includes a serine repeat and a region of alternating acidic and basic amino acids, as well as the variable glutamine repeat.

Protocols

Product Specific Protocols
WB protocol for ATN1 antibody 19243-1-APDownload protocol
IHC protocol for ATN1 antibody 19243-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols