Tested Applications
| Positive WB detected in | mouse brain tissue, fetal human brain tissue, mouse heart tissue, rat heart tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85916-1-RR targets ATP1B1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg3007 Product name: Recombinant Mouse ATP1B1 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 63-304 aa of NM_009721.6 Sequence: ELKPTYQDRVAPPGLTQIPQIQKTEISFRPNDPKSYEAYVLNIIRFLEKYKDSAQKDDMIFEDCGNVPSEPKERGDINHERGERKVCRFKLDWLGNCSGLNDDSYGYREGKPCIIIKLNRVLGFKPKPPKNESLETYPLMMKYNPNVLPVQCTGKRDEDKDKVGNIEYFGMGGYYGFPLQYYPYYGKLLQPKYLQPLLAVQFTNLTVDTEIRVECKAYGENIGYSEKDRFQGRFDVKIEIKS Predict reactive species |
| Full Name | ATPase, Na+/K+ transporting, beta 1 polypeptide |
| Calculated Molecular Weight | 35 kDa |
| Observed Molecular Weight | 40-50 kDa |
| GenBank Accession Number | NM_009721.6 |
| Gene Symbol | Atp1b1 |
| Gene ID (NCBI) | 11931 |
| RRID | AB_3744471 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P14094 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ATP1B1 is one of beta subunits of the Na+/K+ ATPase and responsible for formation and structural integrity of the Na+/K+ ATPase. The Na+/K+ ATPase is a plasma membrane pump consisting of alpha, beta, and gamma subunits. At least four of Na+/K+-ATPase beta subunits (β1, β2, β3, β4) have been identified in mammalian cells; the β1-subunit (ATP1B1) is the most ubiquitous. The Na+/K+ ATPase β subunits have multiple N-glycosylation sites. The predicted MW of ATP1B1 is 35 kDa, while it migrates around 40-52 kDa due to the variable glycosylation. (PMID: 10896885, 17714085)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ATP1B1 antibody 85916-1-RR | Download protocol |
| WB protocol for ATP1B1 antibody 85916-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ATP1B1 Recombinant monoclonal antibody (243093E3), catalog number 85916-1-RR, has 1 citation to date. The cited publication appeared in Journal of Cellular and Molecular Medicine (J Cell Mol Med). The research focuses on epithelial ovarian cancer progression, specifically examining how CRB2 induces polarity changes through activation of the Wnt/PCP signaling pathway. The antibody was used in Western blot (WB) experiments on human samples.
| Species | Application | Title |
|---|---|---|
J Cell Mol Med CRB2 Facilitates Epithelial Ovarian Cancer Progression by Inducing Polarity Changes via Activation of the Wnt/PCP Signalling Pathway. |
