BDNF Monoclonal antibody

BDNF Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1E5C5

Cat no : 66292-1-Ig

Synonyms

Abrineurin, BDNF



Tested Applications

Positive WB detected inU-251 cells, fetal human brain tissue
Positive IF detected inU-251 cells, PC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66292-1-Ig targets BDNF in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, zebrafish
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen BDNF fusion protein Ag11329
Full Name brain-derived neurotrophic factor
Calculated Molecular Weight 247 aa, 28 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC029795
Gene Symbol BDNF
Gene ID (NCBI) 627
RRIDAB_2881675
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

1) What is BDNF?

BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.

2) FAQs for BDNF

A) I can detect more than one band in my samples
BDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).

Protocols

Product Specific Protocols
WB protocol for BDNF antibody 66292-1-IgDownload protocol
IF protocol for BDNF antibody 66292-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cancer Lett

Rab8A promotes breast cancer progression by increasing surface expression of Tropomyosin-related kinase B.

Authors - Yansong Liu
IF

Int J Mol Sci

Methotrexate Neurotoxicity Is Related to Epigenetic Modification of the Myelination Process.

Authors - Yu-Chieh Chen
WB

ACS Chem Neurosci

Quercitrin Rapidly Alleviated Depression-like Behaviors in Lipopolysaccharide-Treated Mice: The Involvement of PI3K/AKT/NF-κB Signaling Suppression and CREB/BDNF Signaling Restoration in the Hippocampus

Authors - Yan Sun
zebrafishWB

Chemosphere

Perfluorooctane sulfonates induces neurobehavioral changes and increases dopamine neurotransmitter levels in zebrafish larvae.

Authors - Luyin Wu
WB

Int Immunopharmacol

Baicalein ameliorates Alzheimer's disease via orchestration of CX3CR1/NF-κB pathway in a triple transgenic mouse model

Authors - Xin-Mei Xie
WB

Exp Neurol

DPP-4 inhibition by linagliptin ameliorates age-related mild cognitive impairment by regulating microglia polarization in mice

Authors - Fen Zhuge