BDNF Monoclonal antibody

BDNF Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1E5C5

Cat no : 66292-1-Ig

Synonyms

Abrineurin, BDNF



Tested Applications

Positive WB detected inU-251 cells, fetal human brain tissue
Positive IF detected inPC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66292-1-Ig targets BDNF in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity rat, zebrafish
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen BDNF fusion protein Ag11329
Full Name brain-derived neurotrophic factor
Calculated molecular weight 247 aa, 28 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC029795
Gene symbol BDNF
Gene ID (NCBI) 627
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

1) What is BDNF?

BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.

2) FAQs for BDNF

A) I can detect more than one band in my samples
BDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).

Protocols

Product Specific Protocols
WB protocol for BDNF antibody 66292-1-IgDownload protocol
IF protocol for BDNF antibody 66292-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
zebrafishWB

Fish Shellfish Immunol

Activation of BDNF-TrkB signaling pathway-regulated brain inflammation in pentylenetetrazole-induced seizures in zebrafish.

Authors - Meng Jin
ratWB

Exp Ther Med

Influencing mechanism of magnolol on expression of BDNF and Bax in rats with cerebral ischemic stroke.

Authors - Zhenbo Liu
ratIF

J Chem Neuroanat

PMID: 32315740

Authors - Li Zhou
ratWB

Brain Res

Electroacupuncture promotes axonal regrowth by attenuating the myelin-associated inhibitors-induced RhoA/ROCK pathway in cerebral ischemia/reperfusion rats.

Authors - Suhui Chen
ratWB

Neurochem Res

Radix Scutellariae Attenuates CUMS-Induced Depressive-Like Behavior by Promoting Neurogenesis via cAMP/PKA Pathway.

Authors - Ruyi Zhang
ratWB

Brain Res

Response of the maternal hypothalamus to cold stress during late pregnancy in rats.

Authors - Di Wang