|Positive WB detected in||U-251 cells, fetal human brain tissue|
|Positive IF detected in||PC-12 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66292-1-Ig targets BDNF in WB, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||rat, zebrafish|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||BDNF fusion protein Ag11329|
|Full Name||brain-derived neurotrophic factor|
|Calculated molecular weight||247aa,28 kDa|
|Observed molecular weight||32 kDa|
|GenBank accession number||BC029795|
|Gene ID (NCBI)||627|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
1) What is BDNF?
BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.
2) FAQs for BDNF
A) I can detect more than one band in my samples
BDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).
Fish Shellfish Immunol
Activation of BDNF-TrkB signaling pathway-regulated brain inflammation in pentylenetetrazole-induced seizures in zebrafish.
Exp Ther Med
Influencing mechanism of magnolol on expression of BDNF and Bax in rats with cerebral ischemic stroke.
J Chem Neuroanat
Colocalization of dopamine receptors in BDNF-expressing peptidergic neurons in the paraventricular nucleus of rats.
Radix Scutellariae Attenuates CUMS-Induced Depressive-Like Behavior by Promoting Neurogenesis via cAMP/PKA Pathway.
Response of the maternal hypothalamus to cold stress during late pregnancy in rats.
Behav Brain Res
Prenatal cold stress: Effect on maternal hippocampus and offspring behavior in rats.