|Positive WB detected in||human heart tissue, mouse brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||PC-3 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
19087-1-AP targets BMPR2 in WB, IP, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||BMPR2 fusion protein Ag5868|
|Full Name||bone morphogenetic protein receptor, type II (serine/threonine kinase)|
|Calculated molecular weight||115 kDa|
|Observed molecular weight||120 kDa|
|GenBank accession number||BC052985|
|Gene ID (NCBI)||659|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
BMPs (bone morphogenetic protein) are involved in endochondral bone formation and embryogenesis. BMPR2 encodes a member of the (BMP) receptor family of transmembrane serine/threonine kinases. It is a widely expressed receptor, with high mRNA expression during development in many tissues. Dimer of BMPR2 receptors (70-80 kD) forms a complex with a dimer of type I BMPR1(50-55 kD) in signal transduction. This antibody recognizes endogenous levels of total BMPR2 protein, including 115 kD precursor, 75 kD mature form and 150 kD dimer.
|Product Specific Protocols|
|WB protocol for BMPR2 antibody 19087-1-AP||Download protocol|
|IHC protocol for BMPR2 antibody 19087-1-AP||Download protocol|
|IP protocol for BMPR2 antibody 19087-1-AP||Download protocol|
|FC protocol for BMPR2 antibody 19087-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Bone morphogenetic protein signaling is required for RAD51-mediated maintenance of genome integrity in vascular endothelial cells.
Augmented noncanonical BMP type II receptor signaling mediates the synaptic abnormality of fragile X syndrome.
J Cell Biol
A PINCH-1-Smurf1 signaling axis mediates mechano-regulation of BMPR2 and stem cell differentiation.