CL594-conjugated Bcl-XL Antibody

Bcl-XL Monoclonal Antibody for IF, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF, ELISA

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4C12A6

Cat no : CL594-66020

Synonyms

Apoptosis regulator Bcl X, BCL2L1, DKFZp781P2092



Tested Applications

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66020-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The CL594-66020 immunogen is Fusion Protein CL594-conjugated Bcl-XL protein expressed in E. coli.

Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Bcl-XL fusion protein Ag18037
Full Name BCL2-like 1
Calculated molecular weight 26 kDa
Observed molecular weight 30 kDa
GenBank accession number BC019307
Gene symbol BCL2L1
Gene ID (NCBI) 598
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20oC. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator.

Protocols

Product Specific Protocols
IF protocol for CL594-conjugated Bcl-XL antibody CL594-66020Download protocol
Standard Protocols
Click here to view our Standard Protocols