CoraLite® Plus 488-conjugated Bcl-XL Monoclonal antibody
Bcl-XL Monoclonal Antibody for FC (Intra), IF
Host / Isotype
Mouse / IgG2b
Reactivity
human, dog
Applications
IF, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
4C12A6
Cat no : CL488-66020
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | HeLa cells, MDCK cells |
Positive FC detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of CL488-66020 is Bcl-XL Fusion Protein expressed in E. coli.
Tested Reactivity | human, dog |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | Bcl-XL fusion protein Ag18037 |
Full Name | BCL2-like 1 |
Calculated molecular weight | 26 kDa |
GenBank accession number | BC019307 |
Gene symbol | BCL2L1 |
Gene ID (NCBI) | 598 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 Bcl-XL antibody CL488-66020 | Download protocol |
FC protocol for CL Plus 488 Bcl-XL antibody CL488-66020 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |