CoraLite®488-conjugated Bcl-XL Monoclonal antibody

Bcl-XL Monoclonal Antibody for FC, IF

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF, FC

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

4C12A6

Cat no : CL488-66020

Synonyms

Apoptosis regulator Bcl X, BCL2L1, DKFZp781P2092



Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66020-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL488-66020 is Bcl-XL Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Bcl-XL fusion protein Ag18037
Full Name BCL2-like 1
Calculated molecular weight 26 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC019307
Gene symbol BCL2L1
Gene ID (NCBI) 598
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator.

Protocols

Product Specific Protocols
IF protocol for CL488 Bcl-XL antibody CL488-66020Download protocol
FC protocol for CL488 Bcl-XL antibody CL488-66020Download protocol
Standard Protocols
Click here to view our Standard Protocols