CoraLite®594-conjugated BCL2L1 Monoclonal antibody

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  IF Staining of HeLa using CL594-66020

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL594-66020 (Bcl-XL antibody) at dilution of 1:100.

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  IF Staining of MDCK using CL594-66020

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using CoraLite®594-conjugated Bcl-XL antibody (CL594-66020, Clone: 4C12A6 ) at dilution of 1:200.

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  FC experiment of HeLa using CL594-66020

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Bcl-XL (CL594-66020, Clone:4C12A6) (red), or 0.4 ug . Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

BCL2L1 Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL594-66020

Clone No.4C12A6

Host / Isotype

Mouse / IgG2b

Reactivity

human, canine

Applications

IF/ICC, FC (Intra)

Synonyms

Bcl-xL, 4C12A6, Apoptosis regulator Bcl X, Apoptosis regulator Bcl-X, Bcl2-L-1

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Tested Applications

• Positive IF/ICC detected in: HeLa cells, MDCK cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66020 targets BCL2L1 in IF/ICC, FC (Intra) applications and shows reactivity with human, canine samples.

• Tested Reactivity: human, canine
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18037

  Product name: Recombinant human Bcl-xL protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-233 aa of BC019307

  Sequence: MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGTESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIAAWMATYLNDHLEPWIQENGGWDTFVELYGNNAAAESRKGQERFNRWFLTGMTVAGVVLLGSLFSRK

• Full Name: BCL2-like 1
• Calculated Molecular Weight: 26 kDa
• GenBank Accession Number: BC019307
• Gene Symbol: BCL2L1
• Gene ID (NCBI): 598
• RRID: AB_2883480
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q07817
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator.