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Tested Applications
| Positive WB detected in | HeLa cells, U-87 MG cells, LNCaP cells, K-562 cells, rat pancreas tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84708-1-RR targets C12orf29 in WB, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag35681 Product name: Recombinant human C12orf29 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-150 aa of NM_001009894.2 Sequence: MKRLGSVQRKMPCVFVTEVKEEPSSKREHQPFKVLATETVSHKALDADIYSAIPTEKVDGTCCYVTTYKDQPYLWARLDRKPNKQAEKRFKNFLHSKENPKEFFWNVEEDFKPAPECWIPAKETEQINGNPVPDENGHIPGWVPVEKNNK Predict reactive species |
| Full Name | chromosome 12 open reading frame 29 |
| Calculated Molecular Weight | 37kDa，325aa |
| Observed Molecular Weight | 37 kDa |
| GenBank Accession Number | NM_001009894.2 |
| Gene Symbol | C12orf29 |
| Gene ID (NCBI) | 91298 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8N999 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
C12orf29, also known as RLIG1, is a 37 kDa human protein consisting of 325 amino acids. It catalyzes ATP-dependent RNA ligation via a three-step mechanism involving tandem auto- and RNA AMPylation (PMID: 36792600).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for C12orf29 antibody 84708-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
