Tested Applications
| Positive WB detected in | pig brain tissue, pig cerebellum tissue, rat brain tissue, rat cerebellum tissue, mouse brain tissue, mouse cerebellum tissue, rabbit brain tissue, rat rain tissue |
| Positive IHC detected in | mouse brain tissue, human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66843-1-Ig targets CaMKII alpha in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4736 Product name: Recombinant human CAMK2A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-271 aa of BC040457 Sequence: MATITCTRFTEEYQLFEELGKGAFSVVRRCVKVLAGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLYKQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPSKRITAAEALKHPWI Predict reactive species |
| Full Name | calcium/calmodulin-dependent protein kinase II alpha |
| Calculated Molecular Weight | 478 aa, 54 kDa |
| Observed Molecular Weight | 45-60 kDa |
| GenBank Accession Number | BC040457 |
| Gene Symbol | CaMKII alpha |
| Gene ID (NCBI) | 815 |
| RRID | AB_2882184 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9UQM7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. It is a member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. It has 10 potential isoforms with a molecular mass range from 21 to 63 kDa. This antibody can recognize all the members of the CAMK2 family.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CaMKII alpha antibody 66843-1-Ig | Download protocol |
| IHC protocol for CaMKII alpha antibody 66843-1-Ig | Download protocol |
| WB protocol for CaMKII alpha antibody 66843-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CaMK2 alpha Monoclonal antibody (1E3B3), catalog number 66843-1-Ig, has 10 citations published in journals including Acta Pharm Sin B, Research, Phytomedicine, CNS Neurosci Ther, Aging, Cell Biol Toxicol, Noncoding RNA Res, Exp Neurol, and Brain Res Bull. The associated research fields span neuroscience and neurology, covering epilepsy, working memory, Alzheimer's disease, vascular cognitive impairment, pain modulation, hearing loss, synaptic plasticity, and neuroinflammation.
| Species | Application | Title |
|---|---|---|
Acta Pharm Sin B Acquired pharmacoresistance in temporal lobe epilepsy is driven by Na(v)1.6-mediated subicular hyperexcitability. | ||
Research (Wash D C) Two Molecularly Defined Neuronal Types in the Mammillary Body Govern Different Temporal Periods during Working Memory Maintenance. | ||
Phytomedicine Zexieyin formula alleviates Alzheimer's disease via post-synaptic CaMKII modulating AMPA receptor: Involved in promoting neurogenesis to strengthen synaptic plasticity in mice hippocampus | ||
CNS Neurosci Ther Electroacupuncture Ameliorates Learning and Memory Deficits in Vascular Cognitive Impairment Rats Through Activation of the Supramammillary Nucleus-Dentate Gyrus Circuit. | ||
CNS Neurosci Ther Electroacupuncture Regulates Sympathetic Nerve Through the NTSGlu-RVLM Circuit to Relieve Spontaneous Pain in SNI Rats | ||
Aging (Albany NY) Gastrodin ameliorates learning and memory impairment in rats with vascular dementia by promoting autophagy flux via inhibition of the Ca2+/CaMKII signal pathway. |
