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CaMKII alpha Monoclonal antibody

CaMKII alpha Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1E3B3

Cat no : 66843-1-Ig

Synonyms

CaM kinase II subunit alpha, CaMK II subunit alpha, CAMK2A, CAMK2B, CAMK2D, CAMK2G, CAMKA, KIAA0968

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CAMK2 Monoclonal antibody (66843-1-Ig)

    WB analysis using 66843-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using CAMK2 Monoclonal antibody (66843-1-Ig)

    WB analysis using 66843-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CAMK2 Monoclonal antibody (66843-1-Ig)

    WB analysis of mouse brain using 66843-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig cerebellum tissue using CAMK2 Monoclonal antibody (66843-1-Ig)

    WB analysis of pig cerebellum using 66843-1-Ig

    pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat cerebellum tissue using CAMK2 Monoclonal antibody (66843-1-Ig)

    WB analysis of rat cerebellum using 66843-1-Ig

    rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse cerebellum tissue using CAMK2 Monoclonal antibody (66843-1-Ig)

    WB analysis of mouse cerebellum using 66843-1-Ig

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using CAMK2 Monoclonal antibody (66843-1-Ig)

    WB analysis of pig brain using 66843-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using CAMK2 Monoclonal antibody (66843-1-Ig)

    WB analysis of rat brain using 66843-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CAMK2 Monoclonal antibody (66843-1-Ig)

    IHC staining of mouse brain using 66843-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66843-1-Ig (CAMK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CAMK2 Monoclonal antibody (66843-1-Ig)

    IHC staining of mouse brain using 66843-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66843-1-Ig (CAMK2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using CAMK2 Monoclonal antibody (66843-1-Ig)

    IHC staining of human renal cell carcinoma using 66843-1-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66843-1-Ig (CAMK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using CAMK2 Monoclonal antibody (66843-1-Ig)

    IHC staining of human renal cell carcinoma using 66843-1-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66843-1-Ig (CAMK2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CAMK2 Monoclonal antibody (66843-1-Ig)

    IF Staining of SH-SY5Y using 66843-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CAMK2 antibody (66843-1-Ig, Clone: 1E3B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inpig brain tissue, pig cerebellum tissue, rat brain tissue, rat cerebellum tissue, mouse brain tissue, mouse cerebellum tissue, rabbit brain tissue, rat rain tissue
Positive IHC detected inmouse brain tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66843-1-Ig targets CaMKII alpha in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Cited Reactivityrat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CaMKII alpha fusion protein Ag4736
Full Name calcium/calmodulin-dependent protein kinase II alpha
Calculated Molecular Weight 478 aa, 54 kDa
Observed Molecular Weight 45-60 kDa
GenBank Accession NumberBC040457
Gene Symbol CAMK2A
Gene ID (NCBI) 815
RRIDAB_2882184
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. It is a member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. This antibody can recognize all the members of the CAMK2 family.

Protocols

Product Specific Protocols
WB protocol for CaMKII alpha antibody 66843-1-IgDownload protocol
IHC protocol for CaMKII alpha antibody 66843-1-IgDownload protocol
IF protocol for CaMKII alpha antibody 66843-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Aging (Albany NY)

Gastrodin ameliorates learning and memory impairment in rats with vascular dementia by promoting autophagy flux via inhibition of the Ca2+/CaMKII signal pathway.

Authors - Ting-Ting Chen
View Article