MIP-3-beta Antibody

MIP-3-beta Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat no : 13397-1-AP

Synonyms

Beta chemokine exodus 3, C C motif chemokine 19, CCL19, CK beta 11, CKb11, EBI1 ligand chemokine, ELC, MIP 3 beta, MIP 3b, MIP3B, MIP-3-beta, SCYA19, Small inducible cytokine A19



Tested Applications

Positive WB detected inA549 cells, HepG2 cells
Positive IHC detected inhuman lymphoma tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 2 publications below

Product Information

13397-1-AP targets MIP-3-beta in IF, IHC, WB, ELISA applications and shows reactivity with human samples.

Reactivity human
Cited Species human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MIP-3-beta fusion protein Ag4200
Full Name chemokine (C-C motif) ligand 19
Calculated molecular weight 98aa,12 kDa
Observed molecular weight 12 kDa
GenBank accession number BC027968
Gene symbol CCL19
Gene ID (NCBI) 6363
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

CCL19, also known as MIP-3β, is one of several CC cytokine genes clustered on the p-arm of chromosome 9. CCL19 may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7.

Protocols

Product Specific Protocols
WB protocol for MIP-3-beta antibody 13397-1-APDownload protocol
IHC protocol for MIP-3-beta antibody 13397-1-APDownload protocol
IF protocol for MIP-3-beta antibody 13397-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Ann Rheum Dis

Global chemokine expression in systemic sclerosis (SSc): CCL19 expression correlates with vascular inflammation in SSc skin.

Authors - Allison L Mathes
humanIF

Am J Respir Crit Care Med

Pulmonary lymphoid neogenesis in idiopathic pulmonary arterial hypertension.

Authors - Perros Frédéric F