MIP-3 Beta Polyclonal antibody

MIP-3 Beta Polyclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 13397-1-AP

Synonyms

Beta chemokine exodus 3, C C motif chemokine 19, CCL19, CK beta 11, CKb11, EBI1 ligand chemokine, ELC, MIP 3 beta, MIP 3b, MIP-3 Beta, MIP3B, MIP-3-beta, SCYA19, Small inducible cytokine A19



Tested Applications

Positive WB detected inTHP-1 cells
Positive IF detected inA549 cells
Positive FC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13397-1-AP targets MIP-3 Beta in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MIP-3 Beta fusion protein Ag4200
Full Name chemokine (C-C motif) ligand 19
Calculated Molecular Weight 98 aa, 12 kDa
GenBank Accession NumberBC027968
Gene Symbol CCL19
Gene ID (NCBI) 6363
RRIDAB_2071529
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCL19, also known as MIP-3β, is one of several CC cytokine genes clustered on the p-arm of chromosome 9. CCL19 may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7.

Protocols

Product Specific Protocols
WB protocol for MIP-3 Beta antibody 13397-1-APDownload protocol
IF protocol for MIP-3 Beta antibody 13397-1-APDownload protocol
FC protocol for MIP-3 Beta antibody 13397-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Am J Respir Crit Care Med

Pulmonary lymphoid neogenesis in idiopathic pulmonary arterial hypertension.

Authors - Perros Frédéric F
humanIF

Ann Rheum Dis

Global chemokine expression in systemic sclerosis (SSc): CCL19 expression correlates with vascular inflammation in SSc skin.

Authors - Allison L Mathes
humanIF

Front Immunol

The Impact of Immune Microenvironment on the Prognosis of Pancreatic Ductal Adenocarcinoma Based on Multi-Omics Analysis

Authors - Bing Yang
humanIHC

J Innate Immun

The Gene Signature of Activated M-CSF-Primed Human Monocyte-Derived Macrophages Is IL-10-Dependent

Authors - Víctor D. Cuevas
humanIHC

Front Physiol

Potential biomarkers and immune cell infiltration involved in aortic valve calcification identified through integrated bioinformatics analysis

Authors - Xiaoshuo Lv
humanIHC

Front Oncol

CXCL12, a potential modulator of tumor immune microenvironment (TIME) of bladder cancer: From a comprehensive analysis of TCGA database

Authors - Jinyan Wang