CL594-conjugated CD44 Antibody

CD44 Monoclonal Antibody for IF, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

IF, ELISA

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

6F4H2

Cat no : CL594-60224

Synonyms

CD44, CD44 antigen, CDW44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, MC56, MDU2, MDU3, MIC4, MUTCH I, PGP 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I



Tested Applications

Positive IF detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 60224-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:200
Sample-dependent, check data in validation data gallery

Product Information

The CL594-60224 immunogen is Fusion Protein CL594-conjugated CD44 protein expressed in E. coli.

Reactivity human, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD44 fusion protein Ag7633
Full Name CD44 molecule (Indian blood group)
Calculated molecular weight 742aa,82 kDa
Observed molecular weight
GenBank accession number BC004372
Gene symbol CD44
Gene ID (NCBI) 960
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications. The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Protocols

Product Specific Protocols
IF protocol for CL594-conjugated CD44 antibody CL594-60224Download protocol
Standard Protocols
Click here to view our Standard Protocols