Tested Applications
| Positive WB detected in | RAW 264.7 cells, mouse skin tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85911-5-RR targets CLEC10A/CD301 in WB, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg2698 Product name: Recombinant Mouse MGL1/CD301a protein (rFc Tag) (HPLC verified) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 57-304 aa of NM_010796.3 Sequence: QNSQLRRDLGTLRATLDNTTSKIKAEFQSLDSRADSFEKGISSLKVDVEDHRQELQAGRDLSQKVTSLESTVEKREQALKTDLSDLTDHVQQLRKDLKALTCQLANLKNNGSEVACCPLHWTEHEGSCYWFSESEKSWPEADKYCRLENSHLVVVNSLEEQNFLQNRLANVVSWIGLTDQNGPWRWVDGTDFEKGFKNWAPLQPDNWFGHGLGGGEDCAHITTGGPWNDDVCQRTFRWICEMKLAKES Predict reactive species |
| Full Name | macrophage galactose N-acetyl-galactosamine specific lectin 1 |
| Calculated Molecular Weight | 35 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | NM_010796.3 |
| Gene Symbol | Mgl1 |
| Gene ID (NCBI) | 17312 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P49300 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
C-type lectin domain family 10 member A (CLEC10A), also known as CD301, macrophage galactose-type C-type lectin (MGL), DC-asialoglyco protein receptor (DC-ASGP-R) or human macrophage lectin (HML), is a type II transmembrane glycoprotein having a calcium-dependent carbohydrate recognition domain (PMID: 8598452; 11919201; 11698450). CLEC10A is expressed exclusively by myeloid professional antigen-presenting cells such as immature dendritic cells (DCs) and alternatively activated macrophages (PMID: 16998493). CLEC10A is a marker for human CD1c+ DCs (cDC2) (PMID: 29755453). It functions as an endocytic receptor for glycosylated antigens (PMID: 11919201).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CLEC10A/CD301 antibody 85911-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
