Calponin Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 13938-1-AP
Basic calponin, Calponin, Calponin 1, Calponin H1, smooth muscle, CNN1, Sm Calp, SMCC
Validation Data Gallery
|Positive WB detected in||mouse heart tissue, mouse lung tissue|
|Positive IP detected in||mouse lung tissue|
|Positive IHC detected in||human heart tissue, human bladder tissue, human breast cancer tissue, human hysteromyoma tissue, human ovary tumor tissue, human skin tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse heart tissue|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13938-1-AP targets Calponin in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Calponin fusion protein Ag4989|
|Full Name||calponin 1, basic, smooth muscle|
|Calculated molecular weight||33 kDa|
|Observed molecular weight||35 kDa|
|GenBank accession number||BC022015|
|Gene ID (NCBI)||1264|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kD protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the full-length of human CNN1 may cross-react with CNN2 and CNN3.
|Product Specific Protocols|
|WB protocol for Calponin antibody 13938-1-AP||Download protocol|
|IHC protocol for Calponin antibody 13938-1-AP||Download protocol|
|IF protocol for Calponin antibody 13938-1-AP||Download protocol|
|IP protocol for Calponin antibody 13938-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Exp Ther Med
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Eur J Pharmacol
Rutaecarpine prevented ox-LDL-induced VSMCs dysfunction through inhibiting overexpression of connexin 43.
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Tanshinone IIA attenuates interleukin-17A-induced systemic sclerosis patient-derived dermal vascular smooth muscle cell activation via inhibition of the extracellular signal-regulated kinase signaling pathway.
Int J Mol Med
In vitro differentiation of endometrial regenerative cells into smooth muscle cells: Α potential approach for the management of pelvic organ prolapse.
Gastroparesis is associated with decreased FOXF1 and FOXF2 in humans, and loss of FOXF1 and FOXF2 results in gastroparesis in mice.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Yajing (Verified Customer) (11-12-2019)
The antibody works well. Can be reused when stored at 4 degrees.
Ying (Verified Customer) (08-19-2019)
very good antibody