Calponin Polyclonal antibody

Calponin Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13938-1-AP

Synonyms

Basic calponin, Calponin, Calponin 1, Calponin H1, smooth muscle, CNN1, Sm Calp, SMCC



Tested Applications

Positive WB detected inmouse heart tissue, mouse lung tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman heart tissue, human bladder tissue, human breast cancer tissue, human hysteromyoma tissue, human ovary tumor tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13938-1-AP targets Calponin in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calponin fusion protein Ag4989
Full Name calponin 1, basic, smooth muscle
Calculated molecular weight 33 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC022015
Gene symbol CNN1
Gene ID (NCBI) 1264
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kD protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the full-length of human CNN1 may cross-react with CNN2 and CNN3.

Protocols

Product Specific Protocols
WB protocol for Calponin antibody 13938-1-APDownload protocol
IHC protocol for Calponin antibody 13938-1-APDownload protocol
IF protocol for Calponin antibody 13938-1-APDownload protocol
IP protocol for Calponin antibody 13938-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Exp Ther Med

Rhynchophylline attenuates allergic bronchial asthma by inhibiting transforming growth factor-β1-mediated Smad and mitogen-activated protein kinase signaling transductions in vivo and in vitro.

Authors - Meng Wang
mouseIF

Redox Biol

Vascular smooth muscle-MAPK14 is required for neointimal hyperplasia by suppressing VSMC differentiation and inducing proliferation and inflammation.

Authors - Wen Wu
ratWB

Eur J Pharmacol

Rutaecarpine prevented ox-LDL-induced VSMCs dysfunction through inhibiting overexpression of connexin 43.

Authors - Meiling Wang
humanIF

Clinics (Sao Paulo)

Tanshinone IIA attenuates interleukin-17A-induced systemic sclerosis patient-derived dermal vascular smooth muscle cell activation via inhibition of the extracellular signal-regulated kinase signaling pathway.

Authors - Mengguo Liu
humanWB

Int J Mol Med

In vitro differentiation of endometrial regenerative cells into smooth muscle cells: Α potential approach for the management of pelvic organ prolapse.

Authors - Xiuhui Chen
mouseWB

Neurogastroenterol Motil

Gastroparesis is associated with decreased FOXF1 and FOXF2 in humans, and loss of FOXF1 and FOXF2 results in gastroparesis in mice.

Authors - Brian Paul Herring

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Yajing (Verified Customer) (11-12-2019)

The antibody works well. Can be reused when stored at 4 degrees.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HPASMC
Calponin Antibody Western Blot, validation (1:1000 dilution) in HPASMC (Cat no:13938-1-AP)
FH

Ying (Verified Customer) (08-19-2019)

very good antibody

  • Applications: Western Blot,
  • Cell Tissue Type: mouse primary cardiomyocytes