Live Chat
  • Featured Product
  • KD/KO Validated

CNPase Monoclonal antibody

CNPase Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Pig, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1B9G9

Cat no : 66729-1-Ig

Synonyms

CNP, CNP1, CNPase

Validation Data Gallery

  • WB analysis using 66729-1-Ig

    WB analysis using 66729-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66729-1-Ig (CNPase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 66729-1-Ig

    WB analysis of HepG2 using 66729-1-Ig

    WB result of CNPase antibody (66729-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNPase transfected HepG2 cells.

  • IF Staining of mouse brain using 66729-1-Ig

    IF Staining of mouse brain using 66729-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 66729-1-Ig (CNPase antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inpig cerebellum tissue, U-251 cells, human brain tissue, HepG2 cells, rat brain tissue, rat cerebellum tissue
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66729-1-Ig targets CNPase in WB, IF, ELISA applications and shows reactivity with Human, Rat, Pig, mouse samples.

Tested Reactivity Human, Rat, Pig, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CNPase fusion protein Ag4227
Full Name 2',3'-cyclic nucleotide 3' phosphodiesterase
Calculated molecular weight 421 aa, 48 kDa
Observed molecular weight 45-48 kDa
GenBank accession numberBC028040
Gene symbol CNP
Gene ID (NCBI) 1267
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CNP(2',3'-cyclic-nucleotide 3'-phosphodiesterase) belongs to the cyclic nucleotide phosphodiesterase family. It plays an essential role in axonal survival but not in myelin assembly, coupling oligodendroglial functions in axonal support and myelination. This protein has 2 isoforms produced by alternative splicing with molecular mass of 45 and 48 kDa.

Protocols

Product Specific Protocols
WB protocol for CNPase antibody 66729-1-IgDownload protocol
IF protocol for CNPase antibody 66729-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols