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CP110 Polyclonal antibody

CP110 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, dog and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 12780-1-AP

Synonyms

Centrosomal protein of 110 kDa, CP110, CP110 protein, KIAA0419



Tested Applications

Positive WB detected inHeLa cells, K-562 cells
Positive IP detected inHeLa cells, multi-cells
Positive IHC detected inhuman testis tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMEF/HeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12780-1-AP targets CP110 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, dog samples.

Tested Reactivity human, mouse, rat, dog
Cited Reactivity human, mouse, rat, Xenopus
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CP110 fusion protein Ag3489
Full Name CP110 protein
Calculated molecular weight 991 aa, 111 kDa
Observed molecular weight 113 kDa
GenBank accession numberBC036654
Gene symbol CP110
Gene ID (NCBI) 9738
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CP110, also named as CCP110 and KIAA0419, is an 110kDa protein. This gene is different to the gene CEP110 (geneID:11064; CNTRL). CP110 is a centriolar protein that positively regulates centrioleduplication while restricting centriole elongation and ciliogenesis. And it acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation.

Protocols

Product Specific Protocols
WB protocol for CP110 antibody 12780-1-APDownload protocol
IHC protocol for CP110 antibody 12780-1-APDownload protocol
IP protocol for CP110 antibody 12780-1-APDownload protocol
FC protocol for CP110 antibody 12780-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Mol Biol Cell

PPP1R35 ensures centriole homeostasis by promoting centriole-to-centrosome conversion.

Authors - Chii Shyang Fong
humanIF

Nat Commun

Investigation of F-BAR domain PACSIN proteins uncovers membrane tubulation function in cilia assembly and transport.

Authors - Christine Insinna
humanIF

Nat Commun

The Cep57-pericentrin module organizes PCM expansion and centriole engagement.

Authors - Koki Watanabe
humanIF

Protein Cell

Neddylation inhibitor MLN4924 suppresses cilia formation by modulating AKT1.

Authors - Hongmei Mao
humanIF

Nat Commun

Self-organization of Plk4 regulates symmetry breaking in centriole duplication.

Authors - Shohei Yamamoto
humanIF

Sci Rep

CEP120 interacts with C2CD3 and Talpid3 and is required for centriole appendage assembly and ciliogenesis.

Authors - Jhih-Jie Tsai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

EK (Verified Customer) (05-04-2020)

works well for immunofluorescence detection of basal body localization

  • Applications: Immunofluorescence