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CPSF7 Recombinant monoclonal antibody (85203-1-RR)

CPSF7 Recombinant monoclonal antibody

CPSF7 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85203-1-RR

Clone No. 242644E1

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

242644E1, CFIm59, Cleavage and polyadenylation specificity factor 59 kDa subunit, Cleavage and polyadenylation specificity factor subunit 7, Cleavage factor Im complex 59 kDa subunit

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIF/ICC

WB analysis using 85203-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 85203-1-RR (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of A431 using 85203-1-RR
Immunofluorescent analysis of (4% PFA) fixed A431 cells using CPSF7 antibody (85203-1-RR, Clone: 242644E1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for CPSF7 in ?

Overview

Tested Applications
Product Information
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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, mouse uterus tissue
Positive IF/ICC detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85203-1-RR targets CPSF7 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	pre-mRNA cleavage factor I, 59 kDa subunit
Calculated Molecular Weight	56 kDa
Observed Molecular Weight	52 kDa
GenBank Accession Number	NM_001136040
Gene Symbol	CPSF7
Gene ID (NCBI)	79869
RRID	AB_3744100
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q8N684
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CPSF7, also named as CPSF59, belongs to the RRM CPSF6/7 family. It probable component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. CPSF7 binds to cleavage and polyadenylation RNA substrates(PMID: 15489334). This antibody is specific to CPSF7.