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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Cytokeratin 19 Monoclonal antibody

Cytokeratin 19 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3G1E4

Cat no : CL488-60187

Synonyms

CK 19, CK19, Cytokeratin 19, Cytoskeleton Marker, K19, K1CS, keratin 19, KRT19

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated Cytokeratin 19 Monoc (CL488-60187)

    IF Staining of HepG2 using CL488-60187

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-60187 (Cytokeratin 19 antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CoraLite® Plus 488-conjugated Cytokeratin 19 Monoc (CL488-60187)

    IF Staining of human breast cancer using CL488-60187

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 488 Cytokeratin 19 antibody (CL488-60187, Clone: 3G1E4 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CoraLite® Plus 488-conjugated Cytokeratin 19 Monoc (CL488-60187)

    IF Staining of human breast cancer using CL488-60187

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 488 Cytokeratin 19 antibody (CL488-60187, Clone: 3G1E4 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated Cytokeratin 19 Monoc (CL488-60187)

    FC experiment of HeLa using CL488-60187

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Cytokeratin 19 (CL488-60187, Clone:3G1E4) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHepG2 cells, human breast cancer tissue
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60187 targets Cytokeratin 19 in IF, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 19 fusion protein Ag7407
Full Name keratin 19
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC002539
Gene Symbol KRT19
Gene ID (NCBI) 3880
RRIDAB_2883114
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients. This antibody, generated against full length KRT19 protein, also recognizes KRT17, another type I keratin homologous to KRT19. KRT17 is used as a marker for trauma.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Cytokeratin 19 antibody CL488-60187Download protocol
Standard Protocols
Click here to view our Standard Protocols