• Featured Product
  • KD/KO Validated

CoraLite®488-conjugated Cytokeratin 19 Monoclonal antibody

Cytokeratin 19 Monoclonal Antibody for FC, IF, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF, FC, ELISA

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

3G1E4

Cat no : CL488-60187

Synonyms

CK 19, CK19, Cytokeratin 19, K19, K1CS, keratin 19, KRT19



Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 60187-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-60187 targets Cytokeratin 19 in IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 19 fusion protein Ag7407
Full Name keratin 19
Calculated molecular weight 44 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC002539
Gene symbol KRT19
Gene ID (NCBI) 3880
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients. This antibody, generated against full length KRT19 protein, also recognizes KRT17, another type I keratin homologous to KRT19. KRT17 is used as a marker for trauma.

Protocols

Product Specific Protocols
IF protocol for CL488-conjugated Cytokeratin 19 antibody CL488-60187Download protocol
FC protocol for CL488-conjugated Cytokeratin 19 antibody CL488-60187Download protocol
Standard Protocols
Click here to view our Standard Protocols