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DCPS Monoclonal antibody

DCPS Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1B2E4

Cat no : 67311-1-Ig

Synonyms

DCPS, DCS 1, DCS1, decapping enzyme, scavenger, HINT 5, HINT5, HSL1, HSPC015

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DCPS Monoclonal antibody (67311-1-Ig)

    WB analysis of HeLa using 67311-1-Ig

    WB result of DCPS antibody (67311-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DCPS transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using DCPS Monoclonal antibody (67311-1-Ig)

    WB analysis using 67311-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67311-1-Ig (DCPS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat spleen tissue using DCPS Monoclonal antibody (67311-1-Ig)

    WB analysis of rat spleen using 67311-1-Ig

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 67311-1-Ig (DCPS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using DCPS Monoclonal antibody (67311-1-Ig)

    WB analysis of mouse brain using 67311-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67311-1-Ig (DCPS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DCPS Monoclonal antibody (67311-1-Ig)

    IHC staining of human breast cancer using 67311-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67311-1-Ig (DCPS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DCPS Monoclonal antibody (67311-1-Ig)

    IHC staining of human breast cancer using 67311-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67311-1-Ig (DCPS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using DCPS Monoclonal antibody (67311-1-Ig)

    IF Staining of human liver cancer using 67311-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using DCPS antibody (67311-1-Ig, Clone: 1B2E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, rat spleen tissue, mouse brain tissue, HepG2 cells, K-562 cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67311-1-Ig targets DCPS in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen DCPS fusion protein Ag8872
Full Name decapping enzyme, scavenger
Calculated Molecular Weight 337 aa, 39 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC014532
Gene Symbol DCPS
Gene ID (NCBI) 28960
RRIDAB_2882571
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DcpS, which is a member of the histidine triad (HIT) superfamily of pyrophosphatases, is the first HIT protein with a defined biological function and identifies the HIT motif as a new mRNA decapping domain. DcpS can encode a 38-40 kDa protein, and has the potential to form a homodimer of 80 kDa(PMID: 12198172).

Protocols

Product Specific Protocols
WB protocol for DCPS antibody 67311-1-IgDownload protocol
IHC protocol for DCPS antibody 67311-1-IgDownload protocol
IF protocol for DCPS antibody 67311-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols