Desmin Antibody
Desmin Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse and More (1)
Applications
IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
3E12C8
Cat no : 60226-1-Ig
Synonyms
DES, desmin
Validation Data Gallery
Tested Applications
| Positive WB detected in | Human heart, tissue |
| Positive IHC detected in | human heart tissue, human hysteromyoma tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 3 publications below |
| IF | See 2 publications below |
Product Information
The 60226-1-Ig immunogen is Fusion Protein Desmin protein expressed in E. coli.
| Reactivity | human, mouse |
| Cited Species | human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Desmin fusion protein Ag9675 |
| Full Name | desmin |
| Calculated molecular weight | 470aa,54 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | BC032116 |
| Gene symbol | DES |
| Gene ID (NCBI) | 1674 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20oC. Aliquoting is unnecessary for -20oC storage. |
Background Information
Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Desmin antibody 60226-1-Ig | Download protocol |
| IHC protocol for Desmin antibody 60226-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochim Biophys Acta Thioredoxin-interacting protein mediates NALP3 inflammasome activation in podocytes during diabetic nephropathy. | ||
J Virol Methods Long-term maintenance of human fetal hepatocytes and prolonged susceptibility to HBV infection by co-culture with non-parenchymal cells. | ||
Reprod Biomed Online Stomatin-like protein 2 is involved in endometrial stromal cell proliferation and differentiation during decidualization in mice and humans. | ||
Diabetes Metab Res Rev Gene expression profiles of rat MMECs with different glucose levels and fgl2 gene silencing. | ||
Br J Pharmacol Catalpol Alleviates Adriamycin-Induced Nephropathy by Activating the SIRT1 Signalling Pathway In Vivo and In Vitro. |
