Desmin Antibody

Desmin Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3E12C8

Cat no : 60226-1-Ig

Synonyms

DES, desmin



Tested Applications

Positive WB detected inHuman heart, tissue
Positive IHC detected inhuman heart tissue, human hysteromyoma tissue, human skeletal muscle tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Sample-dependent, check data in validation data gallery

Product Information

The 60226-1-Ig immunogen is Fusion Protein Desmin protein expressed in E. coli.

Reactivity human, mouse
Cited Species human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Desmin fusion protein Ag9675
Full Name desmin
Calculated molecular weight 470aa,54 kDa
Observed molecular weight 55 kDa
GenBank accession number BC032116
Gene symbol DES
Gene ID (NCBI) 1674
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.

Protocols

Product Specific Protocols
WB protocol for Desmin antibody 60226-1-IgDownload protocol
IHC protocol for Desmin antibody 60226-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochim Biophys Acta

Thioredoxin-interacting protein mediates NALP3 inflammasome activation in podocytes during diabetic nephropathy.

Authors - Pan Gao
humanWB

J Virol Methods

Long-term maintenance of human fetal hepatocytes and prolonged susceptibility to HBV infection by co-culture with non-parenchymal cells.

Authors - Zhou Ming M
humanIF

Reprod Biomed Online

Stomatin-like protein 2 is involved in endometrial stromal cell proliferation and differentiation during decidualization in mice and humans.

Authors - Qian Feng
ratIF

Diabetes Metab Res Rev

Gene expression profiles of rat MMECs with different glucose levels and fgl2 gene silencing.

Authors - Zhenzhong Zheng
mouseWB

Br J Pharmacol

Catalpol Alleviates Adriamycin-Induced Nephropathy by Activating the SIRT1 Signalling Pathway In Vivo and In Vitro.

Authors - Jiangnan Zhang