Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, mouse brain tissue, A549 cells, HCT 116 cells, NIH/3T3 cells, PC-12 cells, rat brain tissue |
| Positive IP detected in | PC-12 cells, rat brain tissue |
| Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
81561-1-RR targets DRP1 (C-terminal) in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag3644 Product name: Recombinant human DNM1L,DLP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 361-711 aa of BC024590 Sequence: CGGARICYIFHETFGRTLESVDPLGGLNTIDILTAIRNATGPRPALFVPEVSFELLVKRQIKRLEEPSLRCVELVHEEMQRIIQHCSNYSTQELLRFPKLHDAIVEVVTCLLRKRLPVTNEMVHNLVAIELAYINTKHPDFADACGLMNNNIEEQRRNRLARELPSAVSRDKLIQDSRRETKNVASGGGGVGDGVQEPTTGNWRGMLKTSKAEELLAEEKSKPIPIMPASPQKGHAVNLLDVPVPVARKLSAREQRDCEVIERLIKSYFLIVRKNIQDSVPKAVMHFLVNHVKDTLQSELVGQLYKSSLLDDLLTESEDMAQRRKEAADMLKALQGASQIIAEIRETHLW Predict reactive species |
| Full Name | dynamin 1-like |
| Calculated Molecular Weight | 736 aa, 82 kDa |
| Observed Molecular Weight | 70-80 kDa |
| GenBank Accession Number | BC024590 |
| Gene Symbol | DRP1 |
| Gene ID (NCBI) | 10059 |
| RRID | AB_2935561 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O00429 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DNM1L(Dynamin-1-like protein) , also known as dynamin-related protein 1 (DRP1), belongs to the dynamin family of large GTPases that mediate membrane remodeling during a variety of cellular processes. DNM1L has an important role in the division of growing mitochondria and peroxisomes and also mediates outer mitochondrial membrane fission in mammalian cells. DNM1L is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain.Variable isoforms of DNM1L have been reported in a tissue-specific manner due to the alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for DRP1 (C-terminal) antibody 81561-1-RR | Download protocol |
| IF protocol for DRP1 (C-terminal) antibody 81561-1-RR | Download protocol |
| IHC protocol for DRP1 (C-terminal) antibody 81561-1-RR | Download protocol |
| IP protocol for DRP1 (C-terminal) antibody 81561-1-RR | Download protocol |
| WB protocol for DRP1 (C-terminal) antibody 81561-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DRP1 (C-terminal) Recombinant monoclonal antibody (2A19), catalog number 81561-1-RR, has a total of 4 citations. These appear in Journal of Ethnopharmacology, Cell Signalling, Fitoterapia, and Biochimica et Biophysica Acta – General Subjects. The associated research fields include diabetic nephropathy and podocyte protection, autophagy and oxidative stress in brain microvascular endothelial cells, psoriasis and mitochondrial homeostasis, and sequestosome 1/heme oxygenase-1 crosstalk in bovine mammary epithelial cells.
| Species | Application | Title |
|---|---|---|
J Ethnopharmacol Senkyunolide a from danggui buxue decoction protects podocytes against diabetic nephropathy via the mir-223-3p/nlrp3 inflammasome axis. | ||
Cell Signal HO-1 enhances autophagy to alleviate oxidative damage in BMECs via Keap1/Nrf2 and AMPK/mTOR signaling.
| ||
Biochim Biophys Acta Gen Subj The crosstalk between sequestosome 1 and heme oxygenase-1 in regulating autophagy/oxidative stress in bovine mammary epithelial cells. | ||
Fitoterapia Kaixuan Jiedu Decoction alleviates imiquimod-induced psoriasis-like skin lesions by inhibiting oxidative stress and enhancing mitochondrial homeostasis through the PI3K/AKT/mTOR pathway. |
