Desmin Monoclonal antibody
Desmin Monoclonal Antibody for IHC, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, pig, mouse
Applications
WB, IHC,ELISA
Conjugate
Unconjugated
CloneNo.
2B1A11
Cat no : 67793-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse testis tissue, pig colon tissue, mouse skeletal muscle tissue |
Positive IHC detected in | human colon tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:20000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Sample-dependent, check data in validation data gallery |
Product Information
67793-1-Ig targets Desmin in WB, IHC,ELISA applications and shows reactivity with Human, pig, mouse samples.
Tested Reactivity | Human, pig, mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Desmin fusion protein Ag9675 |
Full Name | desmin |
Calculated molecular weight | 470 aa, 54 kDa |
Observed molecular weight | 52 kDa |
GenBank accession number | BC032116 |
Gene symbol | DES |
Gene ID (NCBI) | 1674 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.
Protocols
Product Specific Protocols | |
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WB protocol for Desmin antibody 67793-1-Ig | Download protocol |
IHC protocol for Desmin antibody 67793-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |