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Desmin Monoclonal antibody

Desmin Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

2B1A11

Cat no : 67793-1-Ig

Synonyms

CMD1I, CSM1, CSM2, DES, desmin, FLJ12025, FLJ39719, FLJ41013, FLJ41793

Validation Data Gallery

  • WB analysis of mouse testis using 67793-1-Ig

    WB analysis of mouse testis using 67793-1-Ig

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 67793-1-Ig (Desmin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 67793-1-Ig

    IHC staining of human colon using 67793-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67793-1-Ig (Desmin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse testis tissue, pig colon tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman colon tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

67793-1-Ig targets Desmin in WB, IHC,ELISA applications and shows reactivity with Human, pig, mouse samples.

Tested Reactivity Human, pig, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Desmin fusion protein Ag9675
Full Name desmin
Calculated molecular weight 470 aa, 54 kDa
Observed molecular weight52 kDa
GenBank accession numberBC032116
Gene symbol DES
Gene ID (NCBI) 1674
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.

Protocols

Product Specific Protocols
WB protocol for Desmin antibody 67793-1-IgDownload protocol
IHC protocol for Desmin antibody 67793-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols