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  • Western Blot (WB) analysis of various lysates using Di-Methyl-Histone H3 (Lys56) Recombinant antibody (82828-12-RR)

    WB analysis using 82828-12-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82828-12-RR (HIST1H3A(H3K56me2) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using Di-Methyl-Histone H3 (Lys56) Recombinant monoclona (82828-12-RR)

    IF Staining of NIH/3T3 using 82828-12-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Di-Methyl-Histone H3 (Lys56) antibody (82828-12-RR, Clone: 243027A1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Di-Methyl-Histone H3 (Lys56) Recombinant monoclona (82828-12-RR)

    IF Staining of HepG2 using 82828-12-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Di-Methyl-Histone H3 (Lys56) antibody (82828-12-RR, Clone: 243027A1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Dot Blot experiment using Di-Methyl-Histone H3 (Lys56) Recombinant antibody (82828-12-RR)

    Dot Blot experiment using 82828-12-RR

    Dot blot analysis was used to confirm the specificity of 82828-12-RR (Di-Methyl-Histone H3 (Lys56) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

Di-Methyl-Histone H3 (Lys56) Recombinant monoclonal antibody

Di-Methyl-Histone H3 (Lys56) Uni-rAb® Recombinant Antibody for WB, IF/ICC, Dot Blot, ELISA
Cat No. 82828-12-RR
Clone No.243027A1

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Dot Blot, ELISA

Synonyms

Di Methyl Histone H3, Di-Methyl Histone H3 (Lys56), Di-Methyl Histone H3 Lys56, Di-Methyl Histone-H3 Lys56, Di-Methyl-Histone H3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, NIH/3T3 cells, HSC-T6 cells
Positive IF/ICC detected inHepG2 cells, NIH/3T3 cells
Positive Dot Blot detected inpeptide

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82828-12-RR targets Di-Methyl-Histone H3 (Lys56) in WB, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

Predict reactive species
Full Name histone cluster 1, H3a
Observed Molecular Weight15-18 kDa
GenBank Accession NumberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP68431
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.The NSD family histone methyltransferases, including NSD1, NSD2 and NSD3, play crucial roles in chromatin regulation and are implicated in oncogenesis (PMID: 33361816).

Protocols

Product Specific Protocols
IF protocol for Di-Methyl-Histone H3 (Lys56) antibody 82828-12-RRDownload protocol
WB protocol for Di-Methyl-Histone H3 (Lys56) antibody 82828-12-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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