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EPHB1 Monoclonal antibody

EPHB1 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

1B7C12

Cat no : 67080-1-Ig

Synonyms

EK6, ELK, EPH like kinase 6, EPH receptor B1, EPHB1, Ephrin type B receptor 1, EPHT2, FLJ37986, Hek6, NET

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse cerebellum tissue using EPHB1 Monoclonal antibody (67080-1-Ig)

    WB analysis of mouse cerebellum using 67080-1-Ig

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67080-1-Ig (EPHB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using EPHB1 Monoclonal antibody (67080-1-Ig)

    WB analysis of fetal human brain using 67080-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 67080-1-Ig (EPHB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using EPHB1 Monoclonal antibody (67080-1-Ig)

    WB analysis of mouse brain using 67080-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67080-1-Ig (EPHB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse cerebellum tissue, mouse brain tissue, fetal human brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67080-1-Ig targets EPHB1 in WB,ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen EPHB1 fusion protein Ag16347
Full Name EPH receptor B1
Calculated Molecular Weight 984 aa, 110 kDa
Observed Molecular Weight 110-120 kDa
GenBank Accession NumberBC111744
Gene Symbol EPHB1
Gene ID (NCBI) 2047
RRIDAB_2882388
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EPHB1, also named as EPHT2, NET, HEK6 and ELK, belongs to the protein kinase superfamily, Tyr protein kinase family, and Ephrin receptor subfamily. It is a receptor for members of the ephrin-B family. EPHB1 binds to ephrin-B1, -B2 and -B3. EPHB1 may be involved in cell-cell interactions in the nervous system. EPHB1 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Protocols

Product Specific Protocols
WB protocol for EPHB1 antibody 67080-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols