Tested Applications
| Positive WB detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86144-1-RR targets ERCC6 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag18009 Product name: Recombinant human ERCC6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1144-1493 aa of BC127104 Sequence: DESIDEKLGLSYKRERPSQAQTEAFWENKQMENNFYKHKSKTKHHSVAEEETLEKHLRPKQKPKNSKHCRDAKFEGTRIPHLVKKRRYQKQDSENKSEAKEQSNDDYVLEKLFKKSVGVHSVMKHDAIMDGASPDYVLVEAEANRVAQDAPKALRLSRQRCLGAVSGVPTWTGHRGISGAPAGKKSRFGKKRNSNFSVQHPSSTSPTEKCQDGIMKKEGKDNVPEHFSGRAEDADSSSGPLASSSLLAKMRARNHLILPERLESESGHLQEASALLPTTEHDDLLVEMRNFIAFQAHTDGQASTREILQEFESKLSASQSCVFRELLRNLCTFHRTSGGEGIWKLKPEYC Predict reactive species |
| Full Name | excision repair cross-complementing rodent repair deficiency, complementation group 6 |
| Calculated Molecular Weight | 1493 aa, 168 kDa |
| Observed Molecular Weight | 180 kDa |
| GenBank Accession Number | BC127104 |
| Gene Symbol | ERCC6/CSB |
| Gene ID (NCBI) | 2074 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q03468 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ERCC6, also named as CSB, belongs to the SNF2/RAD54 helicase family. It is involved in the preferential repair of active genes. It is presumed DNA or RNA unwinding function. ERCC6 corrects the UV survival and RNA synthesis after UV exposure of Cockayne syndrome complementation group B. Defects in ERCC6 are the cause of Cockayne syndrome type B (CSB) , cerebro-oculo-facio-skeletal syndrome type 1 (COFS1), De Sanctis-Cacchione syndrome (DSC), and UV-sensitive syndrome (UVS). Genetic variation in ERCC6 is associated with susceptibility to age-related macular degeneration type 5 (ARMD5). The antibody is specific to ERCC6.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ERCC6 antibody 86144-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
