Endoglin/CD105 Monoclonal antibody

Endoglin/CD105 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2D5E8

Cat no : 67075-1-Ig

Synonyms

CD105, END, endoglin, ENG, FLJ41744, HHT1, ORW, ORW1



Tested Applications

Positive WB detected inHL-60 cells, HUVEC cells, human placenta tissue
Positive IHC detected inhuman breast cancer tissue, human placenta tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:10000-1:40000
Sample-dependent, check data in validation data gallery

Published Applications

FCSee 1 publications below

Product Information

67075-1-Ig targets Endoglin/CD105 in WB, IHC, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Endoglin/CD105 fusion protein Ag27837
Full Name endoglin
Calculated molecular weight 71 kDa
Observed molecular weight 95-100 kDa
GenBank accession numberBC014271
Gene symbol ENG
Gene ID (NCBI) 2022
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

Protocols

Product Specific Protocols
WB protocol for Endoglin/CD105 antibody 67075-1-IgDownload protocol
IHC protocol for Endoglin/CD105 antibody 67075-1-IgDownload protocol
FC protocol for Endoglin/CD105 antibody 67075-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratFC

J Cell Mol Med

Osthole stimulates bone formation, drives vascularization and retards adipogenesis to alleviate alcohol-induced osteonecrosis of the femoral head.

Authors - Hongping Yu