Validation Data Gallery
|Positive WB detected in||rat liver tissue, L02 cells, HepG2 cells, HT-29 cells, HeLa cells, mouse liver tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells, HepG2 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10624-2-AP targets FASN in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||chicken, human, mosue, mouse, pig, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||FASN fusion protein Ag0975|
|Full Name||fatty acid synthase|
|Calculated molecular weight||272 kDa|
|Observed molecular weight||250-272 kDa|
|GenBank accession number||BC007909|
|Gene ID (NCBI)||2194|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.
|Product Specific Protocols|
|WB protocol for FASN antibody 10624-2-AP||Download protocol|
|IHC protocol for FASN antibody 10624-2-AP||Download protocol|
|IF protocol for FASN antibody 10624-2-AP||Download protocol|
|IP protocol for FASN antibody 10624-2-AP||Download protocol|
|FC protocol for FASN antibody 10624-2-AP||Download protocol|
|Click here to view our Standard Protocols|
Downregulation of PDK4 Increases Lipogenesis and Associates with Poor Prognosis in Hepatocellular Carcinoma.
Cancer Cell Int
Identification of aberrantly methylated differentially expressed genes in prostate carcinoma using integrated bioinformatics.
Biochem Biophys Res Commun
Inhibition of fatty acid synthesis arrests colorectal neoplasm growth and metastasis: Anti-cancer therapeutical effects of natural cyclopeptide RA-XII.
Toxicol Appl Pharmacol
Multi-walled carbon nanotubes promoted lipid accumulation in human aortic smooth muscle cells.
Environ Toxicol Pharmacol
Mono-2-ethylhexyl phthalate induces the expression of genes involved in fatty acid synthesis in HepG2 cells.
Biochim Biophys Acta
Brg1 regulates pro-lipogenic transcription by modulating SREBP activity in hepatocytes.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
K (Verified Customer) (12-20-2020)
I got good results with this Ab for WB at (1:1000) and IHC (1:200)
Iram (Verified Customer) (09-18-2020)
FASN antibody gives a very clean bands.Excellent antibody