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FASN Polyclonal antibody

FASN Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10624-2-AP

Synonyms

FAS, FASN, fatty acid synthase, OA 519, SDR27X1



Tested Applications

Positive WB detected inrat liver tissue, L02 cells, HepG2 cells, HT-29 cells, HeLa cells, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells, HepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10624-2-AP targets FASN in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity chicken, human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FASN fusion protein Ag0975
Full Name fatty acid synthase
Calculated molecular weight 272 kDa
Observed molecular weight 250-272 kDa
GenBank accession numberBC007909
Gene symbol FASN
Gene ID (NCBI) 2194
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.

Protocols

Product Specific Protocols
WB protocol for FASN antibody 10624-2-APDownload protocol
IHC protocol for FASN antibody 10624-2-APDownload protocol
IF protocol for FASN antibody 10624-2-APDownload protocol
IP protocol for FASN antibody 10624-2-APDownload protocol
FC protocol for FASN antibody 10624-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Cancer

Downregulation of PDK4 Increases Lipogenesis and Associates with Poor Prognosis in Hepatocellular Carcinoma.

Authors - Chen Yang
humanIHC

Cancer Cell Int

Identification of aberrantly methylated differentially expressed genes in prostate carcinoma using integrated bioinformatics.

Authors - Kai Wu
humanWB

Biochem Biophys Res Commun

Inhibition of fatty acid synthesis arrests colorectal neoplasm growth and metastasis: Anti-cancer therapeutical effects of natural cyclopeptide RA-XII.

Authors - Yurong Wang
humanWB

Toxicol Appl Pharmacol

Multi-walled carbon nanotubes promoted lipid accumulation in human aortic smooth muscle cells.

Authors - Haiyin Yang
humanWB

Environ Toxicol Pharmacol

Mono-2-ethylhexyl phthalate induces the expression of genes involved in fatty acid synthesis in HepG2 cells.

Authors - Jianying Bai
mouseWB

Biochim Biophys Acta

Brg1 regulates pro-lipogenic transcription by modulating SREBP activity in hepatocytes.

Authors - Nan Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Iram (Verified Customer) (09-18-2020)

FASN antibody gives a very clean bands. Excellent antibody

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000 for western
  • Cell Tissue Type: Human colon cancer