FGFR2 Antibody

FGFR2 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13042-1-AP

Synonyms

BEK, BFR 1, CD332, CEK3, CFD1, ECT1, FGFR 2, FGFR2, JWS, K SAM, KGFR, KSAM, TK14, TK25



Tested Applications

Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

13042-1-AP targets FGFR2 in IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FGFR2 fusion protein Ag3701
Full Name fibroblast growth factor receptor 2
Calculated molecular weight 709aa,79 kDa
Observed molecular weight 120 kDa,85-92 kDa
GenBank accession number BC039243
Gene symbol FGFR2
Gene ID (NCBI) 2263
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FGFR2 (Fibroblast growth factor receptor 2) is a tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis. Ligand binding leads to the activation of several signaling pathway, such as RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP Kinase signaling pathway, as well as the AKT1 signaling pathway. Mutations in the gene of FGFR2 are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis. This polyclonal antibody raised against 358-704aa of human FGFR2 can cross react with other members of the FGFR family.

Protocols

Product Specific Protocols
IHC protocol for FGFR2 antibody 13042-1-APDownload protocol
IF protocol for FGFR2 antibody 13042-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Am J Cancer Res

miR-381-3p suppresses the proliferation of oral squamous cell carcinoma cells by directly targeting FGFR2.

Authors - Xiao Yang