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c-FOS Antibody

c-FOS Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 26192-1-AP

Synonyms

AP 1, C FOS, Cellular oncogene fos, c-FOS, FOS, G0S7, Proto oncogene c Fos



Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

The 26192-1-AP immunogen is Fusion Protein c-FOS protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity dog, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen c-FOS fusion protein Ag24340
Full Name v-fos FBJ murine osteosarcoma viral oncogene homolog
Calculated molecular weight 41 kDa
Observed molecular weight 65 kDa
GenBank accession number BC004490
Gene symbol FOS
Gene ID (NCBI) 2353
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

c-FOS, also named as Proto-oncogene c-Fos and G0/G1 switch regulatory protein 7, is a 380 amino acid protein, which contains 1 bZIP (basic-leucine zipper) domain and belongs to the bZIP family. FOS is expressed at very low levels in quiescent cells. When cells are stimulated to reenter growth, FOS undergo 2 waves of expression, the first one peaks 7.5 minutes following FBS induction. At this stage, the FOS protein is localized endoplasmic reticulum. The second wave of expression occurs at about 20 minutes after induction and peaks at 1 hour. At this stage, the FOS protein becomes nuclear. The calculated molecular weight of FOS is 40 kDa, but Phosphorylated FOS protein is about 60-65 kDa. It is involved in important cellular events, including cell proliferation, differentiation and survival; genes associated with hypoxia; and angiogenesis; which makes its dysregulation an important factor for cancer development. It can also induce a loss of cell polarity and epithelial-mesenchymal transition, leading to invasive and metastatic growth in mammary epithelial cells. Expression of c-fos is an indirect marker of neuronal activity because c-fos is often expressed when neurons fire action potentials. Upregulation of c-fos mRNA in a neuron indicates recent activity

Publications

SpeciesApplicationTitle
dogIF

Int J Cardiol

The effects of interleukin 17A on left stellate ganglion remodeling are mediated by neuroimmune communication in normal structural hearts.

Authors - Jielin Deng
mouseWB

Front Neurosci

Excess Folic Acid Supplementation Before and During Pregnancy and Lactation Activates Fos Gene Expression and Alters Behaviors in Male Mouse Offspring.

Authors - Dandan Chu
humanIF

Oncol Lett

14-3-3ε is a nuclear matrix protein, and its altered expression and localization are associated with curcumin-induced apoptosis of MG-63 cells.

Authors - Kun Lu
mouseWB

Toxicol Res (Camb)

Neurotoxicity, behavioral changes and gene-expression profile of mice exposed to SnS2 nanoflowers.

Authors - Disi Bai
mouseWB

J Cell Biochem

(2R,3R)Dihydromyricetin inhibits osteoclastogenesis and bone loss through scavenging LPS-induced oxidative stress and NF-κB and MAPKs pathways activating.

Authors - Xuejun Zhang
ratIHC

Neuroscience

Peripheral FGFR1 Regulates Myofascial Pain in Rats via the PI3K/AKT Pathway.

Authors - Mingyang Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Matthias (Verified Customer) (09-10-2019)

Good Antibody, very specific.

  • Applications: Immunohistochemistry,Immunofluorescence,
  • Primary Antibody Dilution: 2000
  • Cell Tissue Type: Brain tissue
c-FOS Antibody Immunohistochemistry,Immunofluorescence, validation (2000 dilution) in Brain tissue (Cat no:26192-1-AP)
FH

Elif (Verified Customer) (06-21-2019)

It works better with 2 overnight incubation in 4 degrees.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Dentate gyrus
c-FOS Antibody Immunohistochemistry, validation (1:500 dilution) in Dentate gyrus (Cat no:26192-1-AP)