Galectin-1 Monoclonal antibody

Galectin-1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3G10D2

Cat no : 60223-1-Ig

Synonyms

14 kDa laminin binding protein, 14 kDa lectin, Gal 1, GAL1, Galaptin, Galectin 1, Galectin-1, HBL, HLBP14, HPL, Lactose binding lectin 1, LGALS1, S Lac lectin 1



Tested Applications

Positive WB detected inHepG2 cells, human heart tissue, human placenta tissue, L02 cells, U2OS cells
Positive IHC detected inhuman colon cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 60223-1-Ig is Galectin-1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Galectin-1 fusion protein Ag2432
Full Name lectin, galactoside-binding, soluble, 1
Calculated molecular weight 15 kDa
Observed molecular weight 13 kDa
GenBank accession numberBC020675
Gene symbol LGALS1
Gene ID (NCBI) 3956
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).

Protocols

Product Specific Protocols
WB protocol for Galectin-1 antibody 60223-1-IgDownload protocol
IHC protocol for Galectin-1 antibody 60223-1-IgDownload protocol
IF protocol for Galectin-1 antibody 60223-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Sci Rep

Tissue and plasma levels of galectins in patients with high grade serous ovarian carcinoma as new predictive biomarkers.

Authors - Marilyne Labrie
humanWB

Oncotarget

Design of a peptidic inhibitor that targets the dimer interface of a prototypic galectin.

Authors - Maria Claudia Vladoiu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sushmita (Verified Customer) (12-16-2019)

This Galactin-1 antibody is working very fine which I applied to probe in western blot in cancer cell line.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:4000
  • Cell Tissue Type: Pancreatic cancer cell line
Galectin-1 Antibody Western Blot, validation (1:4000 dilution) in Pancreatic cancer cell line (Cat no:60223-1-Ig)