Tested Applications
| Positive WB detected in | HCT 116 cells, human placenta tissue, human heart tissue, HepG2 cells, U2OS cells, A549 cells, HeLa cells, L02 cells |
| Positive IHC detected in | human colon cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human colon cancer tissue |
| Positive IF/ICC detected in | U2OS cells, Saos-2 cells, human colon cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)-P | IF-P : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60223-1-Ig targets Galectin-1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2432 Product name: Recombinant human Galectin 1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-135 aa of BC020675 Sequence: MACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD Predict reactive species |
| Full Name | lectin, galactoside-binding, soluble, 1 |
| Calculated Molecular Weight | 15 kDa |
| Observed Molecular Weight | 13 kDa |
| GenBank Accession Number | BC020675 |
| Gene Symbol | Galectin-1 |
| Gene ID (NCBI) | 3956 |
| RRID | AB_11182608 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P09382 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Galectin-1 antibody 60223-1-Ig | Download protocol |
| IHC protocol for Galectin-1 antibody 60223-1-Ig | Download protocol |
| WB protocol for Galectin-1 antibody 60223-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Galectin-1 antibody (Cat# 60223-1-Ig) has 11 total citations. These appear in journals including Autophagy, International Journal of Biological Macromolecules, International Journal of Oncology, Journal of Ethnopharmacology, Oncotarget, International Immunopharmacology, mBio, Scientific Reports, iScience, and Journal of Biological Chemistry. Research fields span cancer biology (colorectal, gastric, ovarian, and lung cancer), autophagy, immunology, virology, kidney fibrosis, and ethnopharmacology.
| Species | Application | Title |
|---|---|---|
Autophagy Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy | ||
Int J Biol Macromol Impact of galectin-1's redox state on its lectin activity and monomer-dimer equilibrium. Focusing on oxidized Gal-1 | ||
Int J Oncol Galectin‑1 binds GRP78 to promote the proliferation and metastasis of gastric cancer | ||
J Ethnopharmacol Yang-Xue-An-Tai Decoction Treats URSA by regulating the differentiation of proliferating decidual stromal cells through multiple targets. | ||
Oncotarget Design of a peptidic inhibitor that targets the dimer interface of a prototypic galectin. | ||
Int Immunopharmacol Galectin-1 exacerbates hepatic steatosis by impairing autophagy via interaction with FIP200. |
Reviews
What customers say
Both reviewers rated this antibody 5 stars for Western Blot. One user found it excellent at 1:2000 dilution in ARPE-19 cells but noted no signal in 293T cells. The other reported it worked very well at 1:4000 dilution when probing a pancreatic cancer cell line by Western Blot. Overall, users are satisfied with its performance in cancer cell lines, though results may vary depending on the cell type used.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Guorong (Verified Customer) (05-23-2022) | It is excellent with ARPE-19 cells but gives no signal with 293T
 |
Sushmita (Verified Customer) (12-16-2019) | This Galactin-1 antibody is working very fine which I applied to probe in western blot in cancer cell line.
 |
