CoraLite®594-conjugated Galectin-1 Monoclonal antibody

Galectin-1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3G10D2

Cat no : CL594-60223

Synonyms

14 kDa laminin binding protein, 14 kDa lectin, DKFZp686E23103, Gal 1, GAL1, Galaptin, Galectin 1, Galectin-1, GBP, HBL, HLBP14, HPL, Lactose binding lectin 1, LGALS1, S Lac lectin 1

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Validation Data Gallery

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  IF Staining of human colon cancer using CL594-60223

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 Galectin-1 antibody (CL594-60223, Clone: 3G10D2 ) at dilution of 1:1200.

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  IF Staining of human colon cancer using CL594-60223

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 Galectin-1 antibody (CL594-60223, Clone: 3G10D2 ) at dilution of 1:1200.

• 

  IF Staining of Saos-2 using CL594-60223

  Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using CoraLite®594 Galectin-1 antibody (CL594-60223, Clone: 3G10D2 ) at dilution of 1:200.

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  IF Staining of U2OS using CL594-60223

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CoraLite®594 Galectin-1 antibody (CL594-60223, Clone: 3G10D2 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: human colon cancer tissue, Saos-2 cells, U2OS cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:600-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60223 targets Galectin-1 in IF applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Galectin-1 fusion protein Ag2432
• Full Name: lectin, galactoside-binding, soluble, 1
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC020675
• Gene Symbol: LGALS1
• Gene ID (NCBI): 3956
• RRID: AB_2919903
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).