CoraLite®594-conjugated Galectin-1 Monoclonal antibody

Galectin-1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3G10D2

Cat no : CL594-60223

Synonyms

14 kDa laminin binding protein, 14 kDa lectin, DKFZp686E23103, Gal 1, GAL1, Galaptin, Galectin 1, Galectin-1, GBP, HBL, HLBP14, HPL, Lactose binding lectin 1, LGALS1, S Lac lectin 1



Tested Applications

Positive IF detected inhuman colon cancer tissue, U2OS cells
For other applications, we recommend the unconjugated version of this antibody, 60223-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:600-1:2400
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL594-60223 is Galectin-1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Galectin-1 fusion protein Ag2432
Full Name lectin, galactoside-binding, soluble, 1
Calculated molecular weight 15 kDa
Observed molecular weight 13 kDa
GenBank accession numberBC020675
Gene symbol LGALS1
Gene ID (NCBI) 3956
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm / 614 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).

Protocols

Product Specific Protocols
IF protocol for CL594 Galectin-1 antibody CL594-60223Download protocol
Standard Protocols
Click here to view our Standard Protocols