Tested Applications
| Positive IF-P detected in | human colon cancer tissue |
| Positive IF/ICC detected in | U2OS cells |
| Positive FC (Intra) detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-60223 targets Galectin-1 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2432 Product name: Recombinant human Galectin 1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-135 aa of BC020675 Sequence: MACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD Predict reactive species |
| Full Name | lectin, galactoside-binding, soluble, 1 |
| Calculated Molecular Weight | 15 kDa |
| GenBank Accession Number | BC020675 |
| Gene Symbol | Galectin-1 |
| Gene ID (NCBI) | 3956 |
| RRID | AB_2919238 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P09382 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 Galectin-1 antibody CL488-60223 | Download protocol |
| IF protocol for CL Plus 488 Galectin-1 antibody CL488-60223 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Galectin-1 Monoclonal antibody (3G10D2), catalog number CL488-60223, has 2 citations. These appear in mBio and Scientific Reports. The associated research fields include neuroinfection and extracellular vesicle biology, as well as O-glycosylation in aggressive prostate cancer.
| Species | Application | Title |
|---|---|---|
mBio Herpes simplex virus type 1 modifies the protein composition of extracellular vesicles to promote neurite outgrowth and neuroinfection | ||
Reviews
What customers say
The product received one review with a perfect 5-star rating. The reviewer used it for immunofluorescence on PC3 cells at a 1:200 dilution and included an image demonstrating successful staining. No written comments were provided, but the high rating and shared image indicate satisfaction with the antibody's performance in this application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Margarita (Verified Customer) (06-06-2023) |
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