CoraLite® Plus 488-conjugated Galectin-1 Monoclonal antibody

Galectin-1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3G10D2

Cat no : CL488-60223

Synonyms

14 kDa laminin binding protein, 14 kDa lectin, DKFZp686E23103, Gal 1, GAL1, Galaptin, Galectin 1, Galectin-1, GBP, HBL, HLBP14, HPL, Lactose binding lectin 1, LGALS1, S Lac lectin 1



Tested Applications

Positive IF detected inhuman colon cancer tissue, U2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

The immunogen of CL488-60223 is Galectin-1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Galectin-1 fusion protein Ag2432
Full Name lectin, galactoside-binding, soluble, 1
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 13 kDa
GenBank Accession NumberBC020675
Gene Symbol LGALS1
Gene ID (NCBI) 3956
RRIDAB_2919238
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Galectin-1 antibody CL488-60223Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Sci Rep

The role of GCNT1 mediated O-glycosylation in aggressive prostate cancer

Authors - Kirsty Hodgson

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Margarita (Verified Customer) (06-06-2023)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: PC3 cells
CL Plus 488 Galectin-1 Antibody Immunofluorescence validation (1:200 dilution) in PC3 cells (Cat no:CL488-60223)