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  • Featured Product
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GOSR2/Membrin Monoclonal antibody

GOSR2/Membrin Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

7E7C7

Cat no : 66134-1-Ig

Synonyms

27 kDa Golgi SNARE protein, Bos1, GOSR2, GS27, Membrin

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using GOSR2/Membrin Monoclonal antibody (66134-1-Ig)

    WB analysis of Jurkat using 66134-1-Ig

    WB result of GOSR2/Membrin antibody (66134-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOSR2/Membrin transfected Jurkat cells.

  • Western Blot (WB) analysis of various lysates using GOSR2/Membrin Monoclonal antibody (66134-1-Ig)

    WB analysis using 66134-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66134-1-Ig (GOSR2/Membrin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GOSR2/Membrin Monoclonal antibody (66134-1-Ig)

    WB analysis using 66134-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66134-1-Ig (GOSR2/Membrin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GOSR2/Membrin Monoclonal antibody (66134-1-Ig)

    WB analysis of HeLa using 66134-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66134-1-Ig (GOSR2/Membrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using GOSR2/Membrin Monoclonal antibody (66134-1-Ig)

    IHC staining of human liver using 66134-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66134-1-Ig (GOSR2/Membrin antibody at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human liver tissue using GOSR2/Membrin Monoclonal antibody (66134-1-Ig)

    IHC staining of human liver using 66134-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66134-1-Ig (GOSR2/Membrin antibody at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using GOSR2/Membrin Monoclonal antibody (66134-1-Ig)

    IF Staining of human liver cancer using 66134-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using GOSR2/Membrin antibody (66134-1-Ig, Clone: 7E7C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HSC-T6 cells, Jurkat cells, PC-12 cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 66134-1-Ig is GOSR2/Membrin Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GOSR2/Membrin fusion protein Ag21393
Full Name golgi SNAP receptor complex member 2
Calculated Molecular Weight 212 aa, 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC009710
Gene Symbol GOSR2
Gene ID (NCBI) 9570
RRIDAB_2881533
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Golgi SNAP receptor complex member 2 (GOSR2), also known as GS27 or Membrin, is a member of the solube NSF attachment protein receptor (SNARE) family of vesicle docking proteins. GOSR2 is localized in Golgi apparatus. It is involved in transport of proteins from the cis/medial-Golgi to the trans-Golgi network.

Protocols

Product Specific Protocols
WB protocol for GOSR2/Membrin antibody 66134-1-IgDownload protocol
IHC protocol for GOSR2/Membrin antibody 66134-1-IgDownload protocol
IF protocol for GOSR2/Membrin antibody 66134-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Sci

Neutral sphingomyelinase 2 controls exosome secretion by counteracting V-ATPase-mediated endosome acidification.

Authors - Dolma Choezom
View Article