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  • Western Blot (WB) analysis of HEK-293 cells using GPR101-Specific Polyclonal antibody (20074-1-AP)

    WB analysis of HEK-293 using 20074-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20074-1-AP (GPR101-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COS-7 cells using GPR101-Specific Polyclonal antibody (20074-1-AP)

    WB analysis of COS-7 using 20074-1-AP

    COS-7 cells were subjected to SDS PAGE followed by western blot with 20074-1-AP (GPR101-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using GPR101-Specific Polyclonal antibody (20074-1-AP)

    IHC staining of mouse kidney using 20074-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20074-1-AP (GPR101-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using GPR101-Specific Polyclonal antibody (20074-1-AP)

    IHC staining of mouse kidney using 20074-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20074-1-AP (GPR101-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HEK-293 cells using GPR101-Specific Polyclonal antibody (20074-1-AP)

    FC experiment of HEK-293 using 20074-1-AP

    1X10^6 HEK-293 cells were intracellularly stained with 0.2 ug Anti-Human GPR101-Specific (20074-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

GPR101-Specific Polyclonal antibody

GPR101-Specific Polyclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 20074-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

GPR101, Probable G-protein coupled receptor 101

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inHEK-293 cells, COS-7 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20074-1-AP targets GPR101-Specific in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, monkey samples.

Tested Reactivity human, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

Predict reactive species
Full Name G protein-coupled receptor 101
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberNM_054021
Gene Symbol GPR101
Gene ID (NCBI) 83550
RRIDAB_10665356
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96P66
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPR101, also named as GPCR6, belongs to the G-protein coupled receptor 1 family. It is an orphan receptor. This antibody is specific to GPR101.

Protocols

Product Specific Protocols
IHC protocol for GPR101-Specific antibody 20074-1-APDownload protocol
WB protocol for GPR101-Specific antibody 20074-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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