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Con: 26 μg/150 μl
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HEK-293 cells were subjected to SDS PAGE followed by western blot with 20074-1-AP(GPR101-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours
1X10^6 HEK-293 cells were stained with 0.2ug GPR101-Specific antibody (20074-1-AP, red) and control antibody (blue). Fixed with blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
GPR101, also named as GPCR6, belongs to the G-protein coupled receptor 1 family. It is an orphan receptor. This antibody is specific to GPR101.
COS-7 cells were subjected to SDS PAGE followed by western blot with 20074-1-AP(GPR101-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours
WB protocol for GPR101-Specific antibody 20074-1-AP
FC protocol for GPR101-Specific antibody 20074-1-AP
Standard WB protocol
Standard IHC protocol