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GS28 Polyclonal antibody

GS28 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 16106-1-AP

Synonyms

28 kDa cis Golgi SNARE p28, 28 kDa Golgi SNARE protein, GOLIM2, GOS 28, GOS28, GOS28/P28, GOSR1, GS28, P28

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using GS28 Polyclonal antibody (16106-1-AP)

    WB analysis of NIH/3T3 using 16106-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16106-1-AP (GS28 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using GS28 Polyclonal antibody (16106-1-AP)

    WB analysis of human liver using 16106-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 16106-1-AP (GS28 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using GS28 Polyclonal antibody (16106-1-AP)

    WB analysis of HepG2 using 16106-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16106-1-AP (GS28 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using GS28 Polyclonal antibody (16106-1-AP)

    WB analysis of rat liver using 16106-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 16106-1-AP (GS28 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GS28 Polyclonal antibody (16106-1-AP)

    IHC staining of human stomach cancer using 16106-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16106-1-AP (GS28 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GS28 Polyclonal antibody (16106-1-AP)

    IHC staining of human stomach cancer using 16106-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16106-1-AP (GS28 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GS28 Polyclonal antibody (16106-1-AP)

    IF Staining of HeLa using 16106-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16106-1-AP (GS28 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using GS28 Polyclonal antibody (16106-1-AP)

    FC experiment of HeLa using 16106-1-AP

    1X10^6 HeLa cells were stained with 0.2ug GS28 antibody (16106-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inNIH/3T3 cells, HepG2 cells, human liver tissue, rat liver tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16106-1-AP targets GS28 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GS28 fusion protein Ag9021
Full Name golgi SNAP receptor complex member 1
Calculated Molecular Weight28.6 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC012620
Gene Symbol GOSR1
Gene ID (NCBI) 9527
RRIDAB_2294912
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GS28, also known as GOSR1, is a 28-kDa Golgi SNARE protein that participates in ER-Golgi and intra-Golgi transport. GS28 is considered to be a core component of the Golgi SNARE complex. (PMID: 8638159)

Protocols

Product Specific Protocols
WB protocol for GS28 antibody 16106-1-APDownload protocol
IHC protocol for GS28 antibody 16106-1-APDownload protocol
IF protocol for GS28 antibody 16106-1-APDownload protocol
FC protocol for GS28 antibody 16106-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols