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GSNOR,ADH5 Polyclonal antibody

GSNOR,ADH5 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11051-1-AP

Synonyms

ADH 3, ADH5, ADHX, Alcohol dehydrogenase 5, Alcohol dehydrogenase class 3, FALDH, FDH, GSH FDH, GSNOR, GSNOR,ADH5



Tested Applications

Positive WB detected inmouse kidney tissue, human brain tissue, human heart tissue, mouse brain tissue, mouse liver tissue, human liver tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11051-1-AP targets GSNOR,ADH5 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity chicken, human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSNOR,ADH5 fusion protein Ag1533
Full Name alcohol dehydrogenase 5 (class III), chi polypeptide
Calculated molecular weight 40 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC070491
Gene symbol ADH5
Gene ID (NCBI) 128
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADH5, also named as ADHX, FDH FALDH GSH-FDH ADH3 and GSNOR, belongs to the zinc-containing alcohol dehydrogenase family and Class-III subfamily. It is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. ADH5 mediates multiple cardiovascular functions. It plays in regulating heterocellular communication in the artery wall. (PMID:21071693). ADH5 immunostaining is distributed in both the nucleus and cytoplasm of the retinal ganglion cells(PMID:22117533).

Protocols

Product Specific Protocols
WB protocol for GSNOR,ADH5 antibody 11051-1-APDownload protocol
IHC protocol for GSNOR,ADH5 antibody 11051-1-APDownload protocol
IF protocol for GSNOR,ADH5 antibody 11051-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

Int J Impot Res

S-nitrosylation of endothelial nitric oxide synthase impacts erectile function.

Authors - Parviz K Kavoussi
mouseWB,IHC

J Pharmacol Sci

Increased nitrosoglutathione reductase activity in hypoxic pulmonary hypertension in mice.

Authors - Wu Xiling X
mouseWB

Neurochem Int

Copper disrupts S-nitrosothiol signaling in activated BV2 microglia.

Authors - Alba Rossi-George

Anesth Analg

Treatment with a highly selective β? antagonist causes dose-dependent impairment of cerebral perfusion after hemodilution in rats.

Authors - Hu Tina T
chickenWB,IF

Am J Physiol Cell Physiol

Balance between S-nitrosylation and denitrosylation modulates myoblast proliferation independently of soluble guanylyl cyclase activation.

Authors - Aline M S Yamashita
humanIP

Invest Ophthalmol Vis Sci

S-Nitrosoglutathione inhibits inducible nitric oxide synthase upregulation by redox post-translational modification in experimental diabetic retinopathy.

Authors - Mariana A B Rosales