|Positive WB detected in||Recombinant protein|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Sample-dependent, check data in validation data gallery|
HRP-66001 targets GST Tag in WB applications and shows reactivity with recombinant protein, schistosoma japonicum samples.
|Tested Reactivity||recombinant protein, schistosoma japonicum|
|Host / Isotype||Mouse / IgG2a|
|Full Name||GST Tag|
|Calculated molecular weight||26 kDa|
|Observed molecular weight||-|
|GenBank accession number||FN315687|
|Gene ID (NCBI)|
|Conjugate||HRP Fluorescent Dye|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.|
|Storage Conditions||Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the amino- or carboxy-terminus of targeted proteins. This antibody is conjugated with HRP. Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.
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