HRP-conjugated GST Tag Monoclonal antibody

GST Tag Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2a

Reactivity

recombinant protein, schistosoma japonicum

Applications

WB, IP

Conjugate

HRP Fluorescent Dye

CloneNo.

3G12B10

Cat no : HRP-66001

Synonyms

GST, GST tag



Tested Applications

Positive WB detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:10000-1:100000
Sample-dependent, check data in validation data gallery

Product Information

HRP-66001 targets GST Tag in WB, IP applications and shows reactivity with recombinant protein, schistosoma japonicum samples.

Tested Reactivity recombinant protein, schistosoma japonicum
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Recombinant Protein
Full Name GST Tag
Calculated molecular weight 26 kDa
Observed molecular weight-
GenBank accession numberFN315687
Gene symbol
Gene ID (NCBI)
Conjugate HRP Fluorescent Dye
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the amino- or carboxy-terminus of targeted proteins. This antibody is conjugated with HRP. Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.

Publications

SpeciesApplicationTitle

Nature

Allele-selective lowering of mutant HTT protein by HTT-LC3 linker compounds.

Authors - Zhaoyang Li
WB

Cell Metab

Di-methylation of CD147-K234 Promotes the Progression of NSCLC by Enhancing Lactate Export.

Authors - Ke Wang

Cell Res

Degradation of lipid droplets by chimeric autophagy-tethering compounds.

Authors - Yuhua Fu
WB

Nat Commun

PEBP balances apoptosis and autophagy in whitefly upon arbovirus infection.

Authors - Shifan Wang

J Cell Biol

A PINCH-1-Smurf1 signaling axis mediates mechano-regulation of BMPR2 and stem cell differentiation.

Authors - Ling Guo
WB

Theranostics

NIK links inflammation to hepatic steatosis by suppressing PPARα in alcoholic liver disease.

Authors - Yaru Li