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WB analysis of K-562 using 15874-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 15874-1-AP (Glycophorin A antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 15874-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 15874-1-AP (Glycophorin A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human spleen using 15874-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 15874-1-AP (Glycophorin A antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human spleen using 15874-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 15874-1-AP (Glycophorin A antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human colon cancer using 15874-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15874-1-AP (Glycophorin A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 15874-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15874-1-AP (Glycophorin A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 15874-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15874-1-AP (Glycophorin A antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 15874-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15874-1-AP (Glycophorin A antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human placenta using 15874-1-AP
Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Glycophorin A antibody (15874-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of human placenta using 15874-1-AP
Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Glycophorin A antibody (15874-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Glycophorin A/CD235a Polyclonal antibody

Name: Glycophorin A/CD235a Polyclonal antibody
Brand: Proteintech
SKU: 15874-1-AP

Glycophorin A/CD235a Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 15874-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Synonyms

Glycophorin A, CD235a, Glycophorin-A, GPA, GPErik

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	K-562 cells
Positive IHC detected in	human spleen tissue, human colon cancer tissue, human lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human placenta tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15874-1-AP targets Glycophorin A/CD235a in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8635 Product name: Recombinant human GYPA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 24-150 aa of BC005319 Sequence: EVAMHTSTSSSVTKSYISSQTNDTHKRDTYAATPRAHEVSEISVRTVYPPEEETGERVQLAHHFSEPEITLIIFGVMAGVIGTILLISYGIRRLIKKSPSDVKPLPSPDTDVPLSSVEIENPETSDQ Predict reactive species
Full Name	glycophorin A (MNS blood group)
Calculated Molecular Weight	150 aa, 16 kDa
Observed Molecular Weight	36-38 kDa
GenBank Accession Number	BC005319
Gene Symbol	Glycophorin A
Gene ID (NCBI)	2993
RRID	AB_2116233
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P02724
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)

Protocols

Product Specific Protocols
IF protocol for Glycophorin A/CD235a antibody 15874-1-AP	Download protocol
IHC protocol for Glycophorin A/CD235a antibody 15874-1-AP	Download protocol
WB protocol for Glycophorin A/CD235a antibody 15874-1-AP	Download protocol