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HLA class I ABC Monoclonal antibody

HLA class I ABC Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

5C5B7

Cat no : 66013-1-Ig

Synonyms

HLA A, HLA class I, HLA class I (HLA-A), HLA class I ABC, HLA-A, MHC class I antigen A*3



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, human white blood, U-937 cells, Raji cells, HEK-293 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHepG2 cells, HEK-293 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:10000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66013-1-Ig is HLA class I ABC Fusion Protein expressed in E. coli.

Tested Reactivity human, pig
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HLA class I ABC fusion protein Ag7488
Full Name major histocompatibility complex, class I, A
Calculated molecular weight 41 kDa
Observed molecular weight 40-44 kDa
GenBank accession numberBC003069
Gene symbol HLA-A
Gene ID (NCBI) 3105
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)

Protocols

Product Specific Protocols
WB protocol for HLA class I ABC antibody 66013-1-IgDownload protocol
IHC protocol for HLA class I ABC antibody 66013-1-IgDownload protocol
FC protocol for HLA class I ABC antibody 66013-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Tissue Eng Regen Med

Sequential hydrophile and lipophile solubilization as an efficient method for decellularization of porcine aortic valve leaflets: structure, mechanical property and biocompatibility study.

Authors - Wei-Hua Qiao
humanWB

Oncoimmunology

CD80 expression promotes immune surveillance in Barrett's metaplasia.

Authors - Melania Scarpa
humanIHC

Asian Pac J Cancer Prev

Loss of heterozygosity at 6p21 and HLA class I expression in esophageal squamous cell carcinomas in China.

Authors - Zhao Xiaogang X
humanWB

J Biol Chem

The transferrin receptor CD71 regulates type II CD38, revealing tight topological compartmentalization of intracellular cyclic ADP-ribose production.

Authors - Qi Wen Deng
  • KD Validated

Oncotarget

Combination epidermal growth factor receptor variant III peptide-pulsed dendritic cell vaccine with miR-326 results in enhanced killing on EGFRvIII-positive cells.

Authors - Jianlong Li