HO-1/Hmox1 Recombinant monoclonal antibody

HO-1/Hmox1 Uni-rAb^® Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 83291-3-RR

Clone No.240236G4

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

Hmox1, HO-1, 240236G4, D8Wsu38e, heme oxygenase (decycling) 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIHCFC (Intra)Affinity

WB analysis using 83291-3-RR
Various lysates were subjected to SDS PAGE followed by western blot with 83291-3-RR (HO-1/Hmox1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 83291-3-RR
Various lysates were subjected to SDS PAGE followed by western blot with 83291-3-RR (HO-1/Hmox1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of mouse spleen using 83291-3-RR
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83291-3-RR (HO-1/Hmox1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 83291-3-RR
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83291-3-RR (HO-1/Hmox1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of NIH/3T3 using 83291-3-RR
1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug HO-1/Hmox1 Recombinant antibody (83291-3-RR, Clone:240236G4) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
Affinity and Kinetic Characterization of 83291-3-RR
Biolayer interferometry (BLl) kinetic assays of 83291-3-RR against Mouse HO-1/Hmox1 were performed. The affinity constant is 1.23 nM.

Positive WB detected in	C2C12 cells, NR8383 cells, NIH/3T3 cells, RAW 264.7 cells, 4T1 cells, mouse liver tissue, mouse kidney tissue, mouse spleen tissue
Positive IHC detected in	mouse spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	NIH/3T3 cells

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

83291-3-RR targets HO-1/Hmox1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity	mouse, rat
Cited Reactivity	mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg0938 Product name: Recombinant mouse HO-1/Hmox1 protein Source: mammalian cells-derived, pHZ-KIsec-C-6HIS Tag: C-6HIS Domain: 1-266 aa of NM_010442.2 Sequence: MERPQPDSMPQDLSEALKEATKEVHIQAENAEFMKNFQKGQVSREGFKLVMASLYHIYTALEEEIERNKQNPVYAPLYFPEELHRRAALEQDMAFWYGPHWQEIIPCTPATQHYVKRLHEVGRTHPELLVAHAYTRYLGDLSGGQVLKKIAQKAMALPSSGEGLAFFTFPNIDSPTKFKQLYRARMNTLEMTPEVKHRVTEEAKTAFLLNIELFEELQVMLTEEHKDQSPSQMASLRQRPASLVQDTAPAETPRGKPQISTSSSQT Predict reactive species
Full Name	heme oxygenase (decycling) 1
Calculated Molecular Weight	33 kDa
Observed Molecular Weight	33 kDa
GenBank Accession Number	NM_010442.2
Gene Symbol	HO-1
Gene ID (NCBI)	15368
RRID	AB_3670958
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P14901
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
FC protocol for HO-1/Hmox1 antibody 83291-3-RR	Download protocol
IHC protocol for HO-1/Hmox1 antibody 83291-3-RR	Download protocol
WB protocol for HO-1/Hmox1 antibody 83291-3-RR	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
rat	WB	Phytomedicine Mechanisms of naozhenning in inhibiting neuronal ferroptosis and alleviating brain recurrent mild traumatic brain injury in a rat model. Authors - Qian Wang View Article
mouse	WB	Theranostics Inflammation Cascade-Directed Therapy by Biomimetic Polydopamine Nanosystem for Long-Term Management of Ischemic Stroke. Authors - Yuyi Zheng View Article
mouse	WB	Psychopharmacology (Berl) Xanthouhumol relieves stress-induced depressive-like behaviors through the Sirt1/NF-κB/NLRP3 pathway Authors - Gangqiang Lin View Article