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HRPT2; CDC73 Antibody

HRPT2; CDC73 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, Mouse, Rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4B4H2

Cat no : 66490-1-Ig

Synonyms

C1orf28, CDC73, HPT JT, HRPT2, Hyperparathyroidism 2 protein, Parafibromin



Tested Applications

Positive WB detected inHeLa cells, pig brain tissue, mouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

66490-1-Ig targets HRPT2; CDC73 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

Tested Reactivity Human, Pig, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HRPT2; CDC73 fusion protein Ag2959
Full Name cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
Calculated molecular weight 531aa,61 kDa
Observed molecular weight 61 kDa
GenBank accession number BC014351
Gene symbol CDC73
Gene ID (NCBI) 79577
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HRPT2; CDC73 antibody 66490-1-IgDownload protocol
IHC protocol for HRPT2; CDC73 antibody 66490-1-IgDownload protocol
IF protocol for HRPT2; CDC73 antibody 66490-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IHC

Horm Res Paediatr

A Familial Case of a Whole Germline CDC73 Deletion Discordant for Primary Hyperparathyroidism.

Authors - Naomi Hatabu