• Featured Product
  • KD/KO Validated

IDH2 Antibody

IDH2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F10A10

Cat no : 66918-1-Ig

Synonyms

ICD M, IDH, IDH2, IDHM, IDP, IDPM, mNADP IDH, NADP(+) specific ICDH, Oxalosuccinate decarboxylase



Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, HepG2 cells, SH-SY5Y cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

66918-1-Ig targets IDH2 in WB, ELISA applications and shows reactivity with Human samples.

Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IDH2 fusion protein Ag8950
Full Name isocitrate dehydrogenase 2 (NADP+), mitochondrial
Calculated molecular weight 452aa,51 kDa
Observed molecular weight 45 kDa
GenBank accession number BC009244
Gene symbol IDH2
Gene ID (NCBI) 3418
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IDH2, also named as IDP and ICD-M, belongs to the isocitrate and isopropylmalate dehydrogenases family. It plays a role in intermediary metabolism and energy production. IDH2 is a mitochondrial NADP-dependent isocitrate dehydrogenase that catalyzes oxidative decarboxylation of isocitrate to alpha-ketoglutarate, producing NADPH. It may tightly associate or interact with the pyruvate dehydrogenase complex. IDH1 and IDH2 mutations could also contribute to tumorigenesis and cancer progression through increased mutagenesis. IDH2 has 2 isoforms with the MW of 51 kDa and 45 kDa, and the mature form is about 47 kDa and 41 kDa with the N-terminal transit peptide cleaved.

Protocols

Product Specific Protocols
WB protocol for IDH2 antibody 66918-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols