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  • Featured Product
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IDH2 Monoclonal antibody

IDH2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F10A10

Cat no : 66918-1-Ig

Synonyms

ICD M, IDH, IDH2, IDHM, IDP, IDPM, mNADP IDH, NADP(+) specific ICDH, Oxalosuccinate decarboxylase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IDH2 Monoclonal antibody (66918-1-Ig)

    WB analysis using 66918-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66918-1-Ig (IDH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using IDH2 Monoclonal antibody (66918-1-Ig)

    WB analysis of HEK-293 using 66918-1-Ig

    WB result of IDH2 antibody (66918-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IDH2 transfected HEK-293 cells.

  • Western Blot (WB) analysis of SH-SY5Y cells using IDH2 Monoclonal antibody (66918-1-Ig)

    WB analysis of SH-SY5Y using 66918-1-Ig

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66918-1-Ig (IDH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using IDH2 Monoclonal antibody (66918-1-Ig)

    WB analysis of HepG2 using 66918-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66918-1-Ig (IDH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, HepG2 cells, SH-SY5Y cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66918-1-Ig targets IDH2 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IDH2 fusion protein Ag8950
Full Name isocitrate dehydrogenase 2 (NADP+), mitochondrial
Calculated Molecular Weight 452 aa, 51 kDa
Observed Molecular Weight 41-47 kDa
GenBank Accession NumberBC009244
Gene Symbol IDH2
Gene ID (NCBI) 3418
RRIDAB_2882245
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IDH2, also named as IDP and ICD-M, belongs to the isocitrate and isopropylmalate dehydrogenases family. It plays a role in intermediary metabolism and energy production. IDH2 is a mitochondrial NADP-dependent isocitrate dehydrogenase that catalyzes oxidative decarboxylation of isocitrate to alpha-ketoglutarate, producing NADPH. It may tightly associate or interact with the pyruvate dehydrogenase complex. IDH1 and IDH2 mutations could also contribute to tumorigenesis and cancer progression through increased mutagenesis. IDH2 has 2 isoforms with the MW of 51 kDa and 45 kDa, and the mature form is about 47 kDa and 41 kDa with the N-terminal transit peptide cleaved.

Protocols

Product Specific Protocols
WB protocol for IDH2 antibody 66918-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols