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  • KD/KO Validated

IFT20 Polyclonal antibody

IFT20 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, Canine, rat and More (2)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13615-1-AP

Synonyms

hIFT20, IFT20



Tested Applications

Positive WB detected inHEK-293 cells, MDCK cells, mouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells, MDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:800
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13615-1-AP targets IFT20 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, Canine, rat samples.

Tested Reactivity human, mouse, Canine, rat
Cited Reactivityhuman, mouse, rat, canine, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFT20 fusion protein Ag4521
Full Name intraflagellar transport 20 homolog (Chlamydomonas)
Calculated molecular weight 15 kDa
Observed molecular weight 15-18 kDa
GenBank accession numberBC038094
Gene symbol IFT20
Gene ID (NCBI) 90410
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium. IFT particles are multi-subunit complexes that are made up of complex A and complex B. IFT20 is a component of IFT complex B and involved in ciliary process assembly. It is associated with the Golgi complex and plays a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium.

Protocols

Product Specific Protocols
WB protocol for IFT20 antibody 13615-1-APDownload protocol
IHC protocol for IFT20 antibody 13615-1-APDownload protocol
IF protocol for IFT20 antibody 13615-1-APDownload protocol
IP protocol for IFT20 antibody 13615-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

Functional interaction between autophagy and ciliogenesis.

Authors - Pampliega Olatz O
mouseIF

Nat Cell Biol

Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.

Authors - Quanlong Lu
humanIF

Nat Cell Biol

Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.

Authors - Quanlong Lu
humanWB

Sci Adv

An EMT-primary cilium-GLIS2 signaling axis regulates mammogenesis and claudin-low breast tumorigenesis.

Authors - Molly M Wilson
humanIF

Nat Commun

Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.

Authors - Hai-Qing Tu
humanWB

Nat Commun

EGF receptor kinase suppresses ciliogenesis through activation of USP8 deubiquitinase.

Authors - Kousuke Kasahara

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

kes (Verified Customer) (01-17-2022)

I used it for WB(1:1000) and IF for cilia (1:200) and got good results.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human Liver cells
FH

Boyan (Verified Customer) (03-15-2019)

Excellent for IF labelling of the Golgi under PFA fixation.

  • Applications: Immunofluorescence,
  • Cell Tissue Type: MEF