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  • KD/KO Validated

IFT20 Antibody

IFT20 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, dog and More (2)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13615-1-AP

Synonyms

hIFT20, IFT20



Tested Applications

Positive WB detected inHEK-293 cells, mouse pancreas tissue, mouse kidney tissue, mouse lung tissue, mouse testis tissue, MDCK cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells, MDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13615-1-AP targets IFT20 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, dog samples.

Tested Reactivity human, mouse, dog
Cited Reactivity dog, human, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFT20 fusion protein Ag4521
Full Name intraflagellar transport 20 homolog (Chlamydomonas)
Calculated molecular weight 15 kDa
Observed molecular weight 15-18 kDa
GenBank accession number BC038094
Gene symbol IFT20
Gene ID (NCBI) 90410
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium. IFT particles are multi-subunit complexes that are made up of complex A and complex B. IFT20 is a component of IFT complex B and involved in ciliary process assembly. It is associated with the Golgi complex and plays a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium.

Protocols

Product Specific Protocols
WB protocol for IFT20 antibody 13615-1-APDownload protocol
IHC protocol for IFT20 antibody 13615-1-APDownload protocol
IF protocol for IFT20 antibody 13615-1-APDownload protocol
IP protocol for IFT20 antibody 13615-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

JCI Insight

Hypomorphic mutations of TRIP11 cause odontochondrodysplasia.

Authors - Anika Wehrle
humanWB,IF

Cell Death Differ

The intraflagellar transport protein IFT20 controls lysosome biogenesis by regulating the post-Golgi transport of acid hydrolases.

Authors - Francesca Finetti
  • KD Validated
humanWB

Cell Stress

The primary cilium protein folliculin is part of the autophagy signaling pathway to regulate epithelial cell size in response to fluid flow.

Authors - Naïma Zemirli
humanWB

Proc Natl Acad Sci U S A

EMT programs promote basal mammary stem cell and tumor-initiating cell stemness by inducing primary ciliogenesis and Hedgehog signaling.

Authors - Vincent J Guen
  • KO Validated
dogIF

Biochem Biophys Res Commun

Deficiency of primary cilia in kidney epithelial cells induces epithelial to mesenchymal transition.

Authors - Sang Jun Han
humanWB

Biomol Ther (Seoul)

Autophagy Regulates Formation of Primary Cilia in Mefloquine-Treated Cells.

Authors - Ji Hyun Shin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (03-15-2019)

Excellent for IF labelling of the Golgi under PFA fixation.

  • Applications: Immunofluorescence,
  • Cell Tissue Type: MEF
No image submitted with review