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- KD/KO Validated
IFT20 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 13615-1-AP
|Positive WB detected in||HEK-293 cells, mouse pancreas tissue, mouse kidney tissue, mouse lung tissue, mouse testis tissue, MDCK cells|
|Positive IP detected in||mouse testis tissue|
|Positive IHC detected in||human endometrial cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||hTERT-RPE1 cells, MDCK cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
13615-1-AP targets IFT20 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, dog samples.
|Tested Reactivity||human, mouse, dog|
|Cited Reactivity||dog, human, mouse, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IFT20 fusion protein Ag4521|
|Full Name||intraflagellar transport 20 homolog (Chlamydomonas)|
|Calculated molecular weight||15 kDa|
|Observed molecular weight||15-18 kDa|
|GenBank accession number||BC038094|
|Gene ID (NCBI)||90410|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium. IFT particles are multi-subunit complexes that are made up of complex A and complex B. IFT20 is a component of IFT complex B and involved in ciliary process assembly. It is associated with the Golgi complex and plays a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium.
|Product Specific Protocols|
|WB protocol for IFT20 antibody 13615-1-AP||Download protocol|
|IHC protocol for IFT20 antibody 13615-1-AP||Download protocol|
|IF protocol for IFT20 antibody 13615-1-AP||Download protocol|
|IP protocol for IFT20 antibody 13615-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Hypomorphic mutations of TRIP11 cause odontochondrodysplasia.
Cell Death Differ
The intraflagellar transport protein IFT20 controls lysosome biogenesis by regulating the post-Golgi transport of acid hydrolases.
The primary cilium protein folliculin is part of the autophagy signaling pathway to regulate epithelial cell size in response to fluid flow.
Proc Natl Acad Sci U S A
EMT programs promote basal mammary stem cell and tumor-initiating cell stemness by inducing primary ciliogenesis and Hedgehog signaling.
Biochem Biophys Res Commun
Deficiency of primary cilia in kidney epithelial cells induces epithelial to mesenchymal transition.
Biomol Ther (Seoul)
Autophagy Regulates Formation of Primary Cilia in Mefloquine-Treated Cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Boyan (Verified Customer) (03-15-2019)
Excellent for IF labelling of the Golgi under PFA fixation.