IL-4 Polyclonal antibody

IL-4 Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16545-1-AP

Synonyms

B cell stimulatory factor 1, Binetrakin, BSF 1, BSF1, IL 4, IL4, IL-4, interleukin 4, Pitrakinra



Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

16545-1-AP targets IL-4 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-4 fusion protein Ag9871
Full Name interleukin 4
Calculated molecular weight 153 aa, 17 kDa
GenBank accession numberBC070123
Gene symbol IL4
Gene ID (NCBI) 3565
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

What is the molecular weight of IL-4?

IL-4 has a calculated weight of 14.9 kDa.

What cell type produces the IL-4 protein?

Mast cells in peripheral tissues produce the cytokines IL-4 and IL-13, both of which regulate response to environmental allergens. IL-4 in particular is synthesized rapidly following T cell activation to then stimulate downstream anti-inflammatory processes.

What activity does IL-4 stimulate?

IL-4 is a highly polyfunctional cytokine, contributing to the regulation of many cellular and immunological processes. Increases in IL-4 levels stimulate IL-13 production as well as helper T cell and B cell proliferative responses to anti-immunoglobulin antibodies. IL-4 also aids in controlling T helper cell type 2 (Th2) differentiation, driving differentiation of naive T cells to the Th2 pathway.

How do IL-4 levels respond to brain damage?

Ischemic neurons may secrete IL-4 to help decrease proinflammatory activity of macrophages.(PMID: 26269636)

How do IL-4 levels respond to allergens?

Asthmatics express stimulated IL-4 bronchoalveolar lavage during allergen challenges.

Can polymorphisms in the Il4 gene lead to asthma?

Polymorphisms resulting in mutant IL-4 proteins, are associated with asthma risk, development, and severity.

What does the IL-4: IFN-g ratio signify?

IL-4 promotes Th2 differentiation and stability, promoting its own production while inhibiting Th1 cell differentiation. The Th1 pathway, however, produces interferon gamma (IFN-g). The IL-4:IFN-g ratio gives indication as to which pathway is being enhanced or depressed.

PMIDs: 26088754, 26255210, 25819429,10556256


Publications

SpeciesApplicationTitle
humanWB

J Hematol Oncol

Ovatodiolide suppresses colon tumorigenesis and prevents polarization of M2 tumor-associated macrophages through YAP oncogenic pathways.

Authors - Yan-Jiun Huang
ratELISA

Exp Neurol

Photobiomodulation therapy promotes neurogenesis by improving post-stroke local microenvironment and stimulating neuroprogenitor cells.

Authors - Luodan Yang