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IL-4 Antibody 5 Publications

Mouse Monoclonal| Catalog number: 66142-1-Ig |CloneNo.: 1G2C5

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Con: 111 μg/150 μl

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Tested Applications

Applications:
WB, IHC, FC, ELISA
Application not tested? Please see our Guarantee


Images See all

IL-4 Antibody WB Jurkat cells 66142-1-Ig

Jurkat cells were subjected to SDS PAGE followed by western blot with 66142-1-Ig(IL-4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

IL-4 Antibody IHC human tonsillitis tissue 66142-1-Ig

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66142-1-Ig( IL-4 antibody at dilution of 1:50 (under 10x lens)

IL-4 Antibody IHC human tonsillitis tissue 66142-1-Ig

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66142-1-Ig( IL-4 antibody at dilution of 1:50 (under 40x lens)

Species specificity:
human, rat
Positive WB detected in:
Jurkat cells
Positive IHC detected in:
human tonsillitis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG2a
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
IL-4 fusion protein Ag9940
Full name:
interleukin 4
Calculated molecular weight:
153aa,17 kDa
Observed molecular weight:
18 kDa
GenBank accession number:
BC070123
Gene ID (NCBI):
3565
Gene symbol
IL4
Synonyms
B cell stimulatory factor 1, Binetrakin, BSF 1, BSF1, IL 4, IL4, IL-4, interleukin 4, PS1TP5BP1

Background

IL4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It promotes the proliferation and differentiation of antigen presenting cells. IL4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.


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