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Cytokeratin 19 Monoclonal antibody

Cytokeratin 19 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3G1E4

Cat no : 60187-1-Ig

Synonyms

CK 19, CK19, Cytokeratin 19, K19, K1CS, keratin 19, KRT19



Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells
Positive IHC detected inhuman colon tissue, human lung cancer tissue, human stomach cancer tissue, human breast cancer tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:51200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

60187-1-Ig targets Cytokeratin 19 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 19 fusion protein Ag7407
Full Name keratin 19
Calculated molecular weight 44 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC002539
Gene symbol KRT19
Gene ID (NCBI) 3880
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients. This antibody, generated against full length KRT19 protein, also recognizes KRT17, another type I keratin homologous to KRT19. KRT17 is used as a marker for trauma.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 19 antibody 60187-1-IgDownload protocol
IHC protocol for Cytokeratin 19 antibody 60187-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Stem Cells

Annexin A3 as a Potential Target for Immunotherapy of Liver Cancer Stem-Like Cells.

Authors - Qiu-Zhong Pan