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  • KD/KO Validated

MAP1B Polyclonal antibody

MAP1B Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 21633-1-AP

Synonyms

MAP 1B, MAP1B, MAP5



Tested Applications

Positive WB detected inmouse cerebellum tissue, human brain tissue
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells, mouse brain tissue
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21633-1-AP is MAP1B Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAP1B fusion protein Ag16255
Full Name microtubule-associated protein 1B
Calculated molecular weight 2468 aa, 271 kDa
Observed molecular weight 320 kDa
GenBank accession numberBC141853
Gene symbol MAP1B
Gene ID (NCBI) 4131
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Microtubule-associated protein 1B (MAP1B) is a cytoskeleton protein which can promote microtubule assembly. Previous reports have suggested that this protein is closely involved in neuronal development based on its extensive expression in the developing brain and moderate in mature neurons. Gene disruption or knockout studies of the MAP1B gene led to a delayed development of the nervous system in mice. It includes the N-terminal heavy chain and a C-terminal light chain. The MAP1B heavy chain has a microtubule-stabilization effect, and contains an actin-binding site that may play a role in the crosslinking of actin and microtubules, a function that may be important in neurite elongation. Various isoforms around 300-350 kDa of MAP1B can be observed due to the differences in phosphorylation state. (10704485)

Protocols

Product Specific Protocols
WB protocol for MAP1B antibody 21633-1-APDownload protocol
IHC protocol for MAP1B antibody 21633-1-APDownload protocol
IF protocol for MAP1B antibody 21633-1-APDownload protocol
FC protocol for MAP1B antibody 21633-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Neural Regen Res

GIT1 enhances neurite outgrowth by stimulating microtubule assembly.

Authors - Yi-Sheng Li
humanWB

Cell Cycle

Depletion of JMJD5 sensitizes tumor cells to microtubule-destabilizing agents by altering microtubule stability.

Authors - Junyu Wu
mouseWB

Cereb Cortex

eEF2K/eEF2 Pathway Controls the Excitation/Inhibition Balance and Susceptibility to Epileptic Seizures.

Authors - Christopher Heise
humanWB

EMBO Rep

MAP1B-LC1 prevents autophagosome formation by linking syntaxin 17 to microtubules.

Authors - Kohei Arasaki
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Albane (Verified Customer) (02-18-2020)

A lot of bands, none at the correct size

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: SH-SY5Y
MAP1B Antibody Western Blot, validation (1/1000 dilution) in SH-SY5Y (Cat no:21633-1-AP)