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  • Featured Product
  • KD/KO Validated

c-MYC Polyclonal antibody

c-MYC Polyclonal Antibody for WB, IP, IF, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (6)

Applications

WB, IP, IF, FC, IHC, EMSA, CoIP, chIP, ELISA

Conjugate

Unconjugated

Cat no : 10828-1-AP

Synonyms

bHLHe39, c Myc, c-Myc, MRTL, MYC, Myc proto oncogene protein, Proto oncogene c Myc, Transcription factor p64

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using c-MYC Polyclonal antibody (10828-1-AP)

    WB analysis of A549 using 10828-1-AP

    WB result of c-MYC antibody (10828-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-c-MYC transfected A549 cells.

  • Western Blot (WB) analysis of various lysates using c-MYC Polyclonal antibody (10828-1-AP)

    WB analysis using 10828-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10828-1-AP (c-MYC antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using c-MYC Polyclonal antibody (10828-1-AP)

    WB analysis using 10828-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10828-1-AP (c-MYC antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using c-MYC Polyclonal antibody (10828-1-AP)

    WB analysis using 10828-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10828-1-AP (c-MYC antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using c-MYC Polyclonal antibody (10828-1-AP)

    WB analysis using 10828-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10828-1-AP (c-MYC antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using c-MYC Polyclonal antibody (10828-1-AP)

    WB analysis of human placenta using 10828-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 10828-1-AP (c-MYC antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using c-MYC Polyclonal antibody (10828-1-AP)

    WB analysis of HL-60 using 10828-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 10828-1-AP (c-MYC antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using c-MYC Polyclonal antibody (10828-1-AP)

    IP experiment of MCF-7 using 10828-1-AP

    IP result of anti-c-MYC (IP:10828-1-AP, 4ug; Detection:10828-1-AP 1:1000) with MCF-7 cells lysate 1660 ug.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using c-MYC Polyclonal antibody (10828-1-AP)

    IF Staining of U2OS using 10828-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using c-MYC antibody (10828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using c-MYC Polyclonal antibody (10828-1-AP)

    IF Staining of HEK-293 using 10828-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using c-MYC antibody (10828-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of Saos-2 cells using c-MYC Polyclonal antibody (10828-1-AP)

    IF Staining of Saos-2 using 10828-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Saos-2 cells using c-MYC antibody (10828-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of Saos-2 cells using c-MYC Polyclonal antibody (10828-1-AP)

    IF Staining of Saos-2 using 10828-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Saos-2 cells using c-MYC antibody (10828-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using c-MYC Polyclonal antibody (10828-1-AP)

    FC experiment of HeLa using 10828-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human c-MYC (10828-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inHT-29 cells, Jurkat cells, HeLa cells, A549 cells, HL-60 cells, human placenta tissue, Raji cells, SH-SY5Y cells, HepG2 cells, BxPC-3 cells, SW 1990 cells, Jurkat cells and Raji cells.
Positive IP detected inMCF-7 cells
Positive IF detected inSaos-2 cells, HEK-293 cells, U2OS cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10828-1-AP targets c-MYC in WB, IP, IF, FC, IHC, EMSA, CoIP, chIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, chicken, sheep, P. lilacinum, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen c-MYC fusion protein Ag1263
Full Name v-myc myelocytomatosis viral oncogene homolog (avian)
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 62-65 kDa, 50 kDa
GenBank Accession NumberBC000141
Gene Symbol MYC
Gene ID (NCBI) 4609
RRIDAB_2148585
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Function

c-Myc (also known as Myc), together with l-Myc and n-Myc, belongs to the Myc family of transcription factors. c-Myc has a basic helix-loop-helix leucine zipper domain and through heterodimerization can bind and regulate the transcriptional activity of genes, either by repression or activation. It is a key player in the regulation of cell growth and cell cycle progression and acts as a proto-oncogene.

Tissue specificity

c-Myc is ubiquitously expressed in almost all cell types and its expression positively correlates with tissue proliferative capacity. c-Myc is also expressed during embryogenesis and is upregulated in many cancer types.

Involvement in disease

·         Upregulated in many cancer types, especially in aggressive, poorly differentiated tumors.

·         Mutations in the MYC gene and breakpoint translocations within the MYC gene cause Burkitt lymphoma.

Isoforms

There are 3 different isoforms of c-Myc: c-Myc1, c-Myc2, and c-MycS (PMID: 16260605). They differ in molecular size, can be preferentially expressed during cell growth, and are reported to be functionally distinct.

Post-translational modifications

c-Myc is subject to various post-translational modifications, including phosphorylation, acetylation, and ubiquitinylation (PMID: 16987807), which regulate its activity.

Cellular localization

c-Myc localizes to the nucleus but can also be present in the cytoplasm of certain cancer types.


Protocols

Product Specific Protocols
WB protocol for c-MYC antibody 10828-1-APDownload protocol
IF protocol for c-MYC antibody 10828-1-APDownload protocol
IP protocol for c-MYC antibody 10828-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

RIC-seq for global in situ profiling of RNA-RNA spatial interactions.

Authors - Zhaokui Cai
View Article
mouseWB

Signal Transduct Target Ther

MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration

Authors - Jinpeng Du
  • KD Validated
View Article
humanWB

Signal Transduct Target Ther

Overexpression of CIP2A is associated with poor prognosis in multiple myeloma.

Authors - Xuewen Liu
View Article
humanWB,IF

Gastroenterology

Nucleolar HEATR1 upregulated by mTORC1 signaling promotes hepatocellular carcinoma growth by dominating ribosome biogenesis and proteome homeostasis

Authors - Xiao-Mei Yang
View Article
humanWB

Cancer Cell

p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.

Authors - Alessandro Carugo
  • KD Validated
View Article
WB

Cell Metab

Efferocytosis induces macrophage proliferation to help resolve tissue injury.

Authors - Brennan D Gerlach
  • KD Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Molly (Verified Customer) (05-02-2023)

The antibody worked well for western blotting following the recommended protocol.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Ewing sarcoma cell lines
FH

Anna-Maria (Verified Customer) (08-13-2019)

This antibody worked as expected for western blotting

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Bladder cancer cell lines
FH

Christine (Verified Customer) (11-13-2018)

The antibody worked better than the Abcam c-Myc antibody. However, I did not get clear nuclear staining but rather a mix of nuclear staining with cytoplasmic staining. This could be due non-optimized conditions.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1 to 300
  • Cell Tissue Type: Colon
FH

Claudio (Verified Customer) (12-14-2017)

We tested the antibody in IHC/IFL on mouse brain tumors that showed c-myc upregulation at the mRNA level.

  • Applications: Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 100
  • Cell Tissue Type: Mouse brain tumors with c-myc upregulation (determined at the mRNA level)