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c-MYC Polyclonal antibody

c-MYC Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IF, FC, CoIP, chIP, ELISA

Conjugate

Unconjugated

Cat no : 10828-1-AP

Synonyms

bHLHe39, c Myc, c-Myc, MRTL, MYC, Myc proto oncogene protein, Proto oncogene c Myc, Transcription factor p64



Tested Applications

Positive WB detected inHepG2 cells, HL-60 cells, human placenta tissue
Positive IP detected inHL-60 cells
Positive IF detected inNCCIT cell
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10828-1-AP targets c-MYC in WB, IP, IF, FC, CoIP, chIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen c-MYC fusion protein Ag1263
Full Name v-myc myelocytomatosis viral oncogene homolog (avian)
Calculated molecular weight 49 kDa
Observed molecular weight 62-65 kDa, 50 kDa
GenBank accession numberBC000141
Gene symbol MYC
Gene ID (NCBI) 4609
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Function

c-Myc (also known as Myc), together with l-Myc and n-Myc, belongs to the Myc family of transcription factors. c-Myc has a basic helix-loop-helix leucine zipper domain and through heterodimerization can bind and regulate the transcriptional activity of genes, either by repression or activation. It is a key player in the regulation of cell growth and cell cycle progression and acts as a proto-oncogene.

Tissue specificity

c-Myc is ubiquitously expressed in almost all cell types and its expression positively correlates with tissue proliferative capacity. c-Myc is also expressed during embryogenesis and is upregulated in many cancer types.

Involvement in disease

·         Upregulated in many cancer types, especially in aggressive, poorly differentiated tumors.

·         Mutations in the MYC gene and breakpoint translocations within the MYC gene cause Burkitt lymphoma.

Isoforms

There are 3 different isoforms of c-Myc: c-Myc1, c-Myc2, and c-MycS (PMID: 16260605). They differ in molecular size, can be preferentially expressed during cell growth, and are reported to be functionally distinct.

Post-translational modifications

c-Myc is subject to various post-translational modifications, including phosphorylation, acetylation, and ubiquitinylation (PMID: 16987807), which regulate its activity.

Cellular localization

c-Myc localizes to the nucleus but can also be present in the cytoplasm of certain cancer types.


Protocols

Product Specific Protocols
WB protocol for c-MYC antibody 10828-1-APDownload protocol
IHC protocol for c-MYC antibody 10828-1-APDownload protocol
IF protocol for c-MYC antibody 10828-1-APDownload protocol
IP protocol for c-MYC antibody 10828-1-APDownload protocol
FC protocol for c-MYC antibody 10828-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Stem Cell Res Ther

Exosomes from glioma cells induce a tumor-like phenotype in mesenchymal stem cells by activating glycolysis.

Authors - Zhanjun Ma
humanWB

Clin Sci (Lond)

PMID: 30782607

Authors - Chao Liu

J Exp Clin Cancer Res

LPCAT1 promotes brain metastasis of lung adenocarcinoma by up-regulating PI3K/AKT/MYC pathway.

Authors - Chunhua Wei

Cancer Med

A novel tumor suppressor SPINK5 targets Wnt/β-catenin signaling pathway in esophageal cancer.

Authors - Qian Wang
humanWB

ACS Appl Mater Interfaces

ROS-Responsive Polymeric Micelles for Triggered Simultaneous Delivery of PLK1 Inhibitor/miR-34a and Effective Synergistic Therapy in Pancreatic Cancer.

Authors - Xiaofei Xin
humanIHC

Oncogene

AKIP1 promotes early recurrence of hepatocellular carcinoma through activating the Wnt/β-catenin/CBP signaling pathway.

Authors - Yanmei Cui

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Anna-Maria (Verified Customer) (08-13-2019)

This antibody worked as expected for western blotting

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Bladder cancer cell lines
FH

Christine (Verified Customer) (11-13-2018)

The antibody worked better than the Abcam c-Myc antibody. However, I did not get clear nuclear staining but rather a mix of nuclear staining with cytoplasmic staining. This could be due non-optimized conditions.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1 to 300
  • Cell Tissue Type: Colon
FH

Claudio (Verified Customer) (12-14-2017)

We tested the antibody in IHC/IFL on mouse brain tumors that showed c-myc upregulation at the mRNA level.

  • Applications: Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 100
  • Cell Tissue Type: Mouse brain tumors with c-myc upregulation (determined at the mRNA level)