MYC tag Monoclonal antibody
MYC tag Monoclonal Antibody for IF, IP, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
recombinant protein and More (1)
Applications
WB, IP, IF, CoIP,ELISA
Conjugate
Unconjugated
CloneNo.
1A5A2
Cat no : 60003-2-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | recombinant protein |
| Positive IP detected in | Transfected HEK-293 cells |
| Positive IF detected in | Transfected HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:2000-1:10000 for WB |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 21 publications below |
| IF | See 1 publications below |
| IP | See 6 publications below |
| CoIP | See 2 publications below |
Product Information
60003-2-Ig targets MYC tag in WB, IP, IF, CoIP,ELISA applications and shows reactivity with recombinant protein samples.
Background Information
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MYC tag antibody 60003-2-Ig | Download protocol |
| IP protocol for MYC tag antibody 60003-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Mol Med The pattern-recognition molecule mindin binds integrin Mac-1 to promote macrophage phagocytosis via Syk activation and NF-κB p65 translocation. | ||
Elife Distinct RanBP1 nuclear export and cargo dissociation mechanisms between fungi and animals. | ||
Proc Natl Acad Sci U S A SENCR stabilizes vascular endothelial cell adherens junctions through interaction with CKAP4. | ||
Commun Biol Phosphoproteomics identifies dual-site phosphorylation in an extended basophilic motif regulating FILIP1-mediated degradation of filamin-C. | ||
Mol Plant Pathol Pm21 CC domain activity modulated by intramolecular interactions is implicated in cell death and disease resistance. | ||
Biomolecules (20S)G-Rh2 Inhibits NF-κB Regulated Epithelial-Mesenchymal Transition by Targeting Annexin A2. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tom (Verified Customer) (02-10-2021) | HEK293T cells were transfected with a Myc-tagged construct. The 60003-2-Ig antibody (1:2000) resulted in a lot of background. An alternative antibody was used alongside this one, with a stronger band and less background being achieved.
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FH Alinda (Verified Customer) (10-09-2019) | This antibody was used at 1:1000 in HEK cells transfected with a myc-tagged insert cloned into an overxpression. Cells were blocked for 1 hr at room temp in 5%goat serum PBST.This antibody has a lot of background; another antibody from cell signalling was tested in parallel and performed much better
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