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MYC tag Antibody 30 Publications

Mouse Monoclonal| Catalog number: 60003-2-Ig |CloneNo.: 1A5A2

1 customer review

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Con: 128 μg/150 μl

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Tested Applications

Applications:
WB, IP, IF, ELISA
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Images See all

MYC tag Antibody WB recombinant proein 60003-2-Ig

Western blot of Myc-tagged fusion protein with anti-Myc-tag (60003-2-Ig) at various dilutions.

MYC tag Antibody IP Transfected HEK-293 cells 60003-2-Ig

IP Result of anti-MYC tag (IP:60003-2-Ig, 4ug; Detection:60003-2-Ig 1:10000) with Transfected HEK-293 cells lysate 300ug.

MYC tag Antibody IF Transfected HEK-293 cells 60003-2-Ig

Immunofluorescent analysis of Transfected HEK-293 cells using 60003-2-Ig(MYC tag antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
recombinant protein, human, mouse
Positive WB detected in:
recombinant protein
Positive IP detected in:
Transfected HEK-293 cells
Positive IF detected in:
Transfected HEK-293 cells
Recommended dilution:
WB : 1:2000-1:10000
IP : 0.5-4.0 ug for IP and 1:2000-1:10000 for WB
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
MYC tag
Calculated molecular weight:
Observed molecular weight:
GenBank accession number:
Gene ID (NCBI):
Gene symbol
Synonyms
EQKLISEEDL, Myc tag

Background

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

MYC tag Mouse Monoclonal antibody

Alinda (Verified Customer), 10-09-2019
Applications
Immunofluorescence,
Primary Antibody Dilution
1:1000
No image submitted with review

No image submitted with review

Cell/Tissue Type
HEK cells
Lot Number
10003656
This antibody was used at 1:1000 in HEK cells transfected with a myc-tagged insert cloned into an overxpression. Cells were blocked for 1 hr at room temp in 5%goat serum PBST. This antibody has a lot of background; another antibody from cell signalling was tested in parallel and performed much better
Proteintech Response
Thank you for your feedback on this item. We will re-examine this product in QC and will be in contact with you about a refund.

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