MYC tag Monoclonal antibody

MYC tag Monoclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1A5A2

Cat no : 60003-2-Ig

Synonyms

EQKLISEEDL, Myc tag



Tested Applications

Positive WB detected inrecombinant protein
Positive IP detected inTransfected HEK-293 cells
Positive IF detected inTransfected HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:2000-1:10000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

60003-2-Ig targets MYC tag in WB, IP, IF, CoIP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name MYC tag
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.

Protocols

Product Specific Protocols
IF protocol for MYC tag antibody 60003-2-IgDownload protocol
IP protocol for MYC tag antibody 60003-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

J Cell Mol Med

The pattern-recognition molecule mindin binds integrin Mac-1 to promote macrophage phagocytosis via Syk activation and NF-κB p65 translocation.

Authors - Yuan-Sheng Liu

Elife

Distinct RanBP1 nuclear export and cargo dissociation mechanisms between fungi and animals.

Authors - Yuling Li
IP

Proc Natl Acad Sci U S A

SENCR stabilizes vascular endothelial cell adherens junctions through interaction with CKAP4.

Authors - Qing Lyu
WB,IP

Commun Biol

Phosphoproteomics identifies dual-site phosphorylation in an extended basophilic motif regulating FILIP1-mediated degradation of filamin-C.

Authors - Lena Reimann
CoIP

Mol Plant Pathol

Pm21 CC domain activity modulated by intramolecular interactions is implicated in cell death and disease resistance.

Authors - Anli Gao
WB,IP

Biomolecules

(20S)G-Rh2 Inhibits NF-κB Regulated Epithelial-Mesenchymal Transition by Targeting Annexin A2.

Authors - Yu-Shi Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Alinda (Verified Customer) (10-09-2019)

This antibody was used at 1:1000 in HEK cells transfected with a myc-tagged insert cloned into an overxpression. Cells were blocked for 1 hr at room temp in 5%goat serum PBST. This antibody has a lot of background; another antibody from cell signalling was tested in parallel and performed much better

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK cells