CoraLite®488-conjugated N-cadherin Monoclonal antibody

N-cadherin Monoclonal Antibody for IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, mouse, rat

Applications

IF, ELISA

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

1D8B3

Cat no : CL488-66219

Synonyms

Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, Neural cadherin



Tested Applications

Positive IF detected inmouse heart tissue
For other applications, we recommend the unconjugated version of this antibody, 66219-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66219 targets N-cadherin in IF, ELISA applications and shows reactivity with human, pig, mouse, rat samples.

Tested Reactivity human, pig, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen N-cadherin fusion protein Ag4996
Full Name cadherin 2, type 1, N-cadherin (neuronal)
Calculated molecular weight 906 aa, 100 kDa
GenBank accession numberBC036470
Gene symbol CDH2
Gene ID (NCBI) 1000
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% ProClin 300,pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Protocols

Product Specific Protocols
IF protocol for CL488-conjugated N-cadherin antibody CL488-66219Download protocol
Standard Protocols
Click here to view our Standard Protocols